FastQCFastQC Report
Wed 25 May 2016
SRR1294967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4825775
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT149320.309421802715626No Hit
GGTATCAACGCAGAGTACTTTTTTT90550.18763825499531162No Hit
TATCAACGCAGAGTACTTTTTTTTT81810.169527174391678No Hit
CCCATGTACTCTGCGTTGATACCAC79770.16529987411348435No Hit
GTACATGGGAAGCAGTGGTATCAAC77900.16142484885847352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT550.003072287712.0897357
CGTATAC750.002646880210.1344493
GTATTAG8450.09.5625181
CAGTGCG900.0011153989.4990789
CTGGTCG950.00182651938.9991269
TGTCCGT950.00182651938.99912610
ATACCGA850.0074346688.9418836
ACCGCTC850.0074454188.9403098
TGTCGCC3800.08.74915110
GTCTCGC3300.08.6420621
TAGACTG6000.08.5506755
CTAGGAC4500.08.4453753
GTCCTAG4850.08.4282371
GTAGGAC3400.08.3832763
CTAGACA6050.08.3229724
TAGGACT5650.08.239594
CTGTCGC4050.08.209089
TCTGTCG3950.08.1764218
GTCTTAG5150.08.121861
TATTAGG8200.08.1118272
Produced by FastQC (version 0.11.5)