FastQCFastQC Report
Wed 25 May 2016
SRR1294966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258712
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC61550.14452726552065506No Hit
CCCATGTACTCTGCGTTGATACCAC55220.12966361660520834No Hit
GTATCAACGCAGAGTACTTTTTTTT47270.11099600066874679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG803.7733614E-410.6875675
CGTACCT750.00264543810.1350644
GCACCGT1304.2530082E-69.4982726
GTCTAGG4600.09.3069921
TATACCG1151.1114211E-49.0870125
TAAACCG850.0074388128.9412325
CTTAGAC3000.08.8678683
GTCTACG1401.066384E-58.8342551
GTCTAGA4850.08.82725051
GTCGTAG1000.0028685698.5624321
GGACCGT1252.7506045E-48.35847956
GTATTAG6500.08.3428821
GTCTTAG4050.08.2218141
TTAGTAC3600.08.18166353
AGTACTG6050.08.165345
TAGACAG7100.08.1620235
CCGGGTT3400.08.10213517
GTCCTGG8700.08.0922091
ATTATAC7550.08.0540713
GTGCTAG4850.08.0426051
Produced by FastQC (version 0.11.5)