FastQCFastQC Report
Wed 25 May 2016
SRR1294966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258712
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT135680.3185939786489436No Hit
GGTATCAACGCAGAGTACTTTTTTT87660.20583688213713444No Hit
TATCAACGCAGAGTACTTTTTTTTT74580.175123370634126No Hit
CCCATGTACTCTGCGTTGATACCAC60700.14253135689851767No Hit
GTACATGGGAAGCAGTGGTATCAAC60670.14246091306479516No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42850.10061727583363232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTG750.00265117410.1324197
GTGTACG900.00110739269.5065091
CTATACG800.0045167229.500374
TAGGACG900.00111395529.500374
TATACTG8550.09.2225825
CGTGCAA1356.862094E-69.14732210
TTACGGG950.00182338919.0007733
TAGACAG7100.08.96513755
TAGACCG850.0074368178.94152455
ACACTCG850.0074368178.94152456
TAAGACA6700.08.7913884
TAGACTG6250.08.6643375
CTTAGAC3200.08.6101153
GTACGGA1000.00288858528.5558581
GGACGAT1000.0029055278.5503336
CTAGACT4900.08.5309454
CTGTGCG2356.184564E-108.4885969
TCTATAC5400.08.4451693
TTATACT7600.08.3753264
GGTATCA43950.08.3492891
Produced by FastQC (version 0.11.5)