FastQCFastQC Report
Wed 25 May 2016
SRR1294965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164988
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCC604.1013455E-412.6641928
CCGTGCG604.102177E-412.6638879
GGTATCA18450.011.0870831
GTCGTAG750.002616410.1485471
AGGATCG750.0026487110.1335445
GTATCAA37300.09.7438311
CGTGCTA800.00452497979.49825810
GTGTAGG4200.09.2877341
TAGGACC2850.08.6688274
TAGGACG1107.1309705E-48.6385164
AACCGTG1107.162588E-48.6346767
AGACCGT1107.162588E-48.6346766
GCGCGGT1000.00291135168.54843113
GTGTTAG6250.08.524781
GTCTCGC2356.002665E-108.5021081
TTCCGTA1356.818847E-58.4427947
CGTAGAC1150.00110862018.262833
CTGGTCG1150.00111364438.2590569
ACCGAGC1502.594544E-58.2317248
TATTCCG2201.4657417E-88.2047175
Produced by FastQC (version 0.11.5)