FastQCFastQC Report
Wed 25 May 2016
SRR1294965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164988
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT120030.2881881052238326No Hit
GGTATCAACGCAGAGTACTTTTTTT71850.17250950062761286No Hit
TATCAACGCAGAGTACTTTTTTTTT65680.1576955323760837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG701.0909581E-412.2152355
GGTATCA23700.012.11267951
CGTCCGC550.00307201312.08981610
CTTACCG550.00307201312.0898169
GACCGTG1355.703987E-810.5546027
CGCACGA909.531692E-510.55460212
GTATCAA50300.010.1670731
CCGGTCG750.002651155510.1324189
ATACCGA800.0045152429.5007396
AGACCGT1252.75396E-59.1207096
GTCTTAG3900.09.0181771
ACGCACG950.00182629778.999186511
TTATACT7500.08.9940334
CCTACAC4250.08.9419783
GAACCGA850.00743439478.9418726
TAGGACC2553.6379788E-128.9418724
AATACCG850.00743439478.9418725
ACCGATC850.00744465138.9403698
GTCTAGA4550.08.7744431
TTACACT6850.08.73790554
Produced by FastQC (version 0.11.5)