FastQCFastQC Report
Wed 25 May 2016
SRR1294964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188809
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC45760.10924346275993965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCT700.001494744810.8550276
TAATCCG750.002648994610.1334155
TTCGGAC951.644801E-410.0003218
GTCCTAG4850.09.6128351
CTAGACA4400.09.5008734
GACCCGT800.00452541759.4981496
ACCGTGT2700.09.4981498
TAGACAG6050.08.9504865
CTACGGA850.0074335458.9419984
CTACACT6850.08.8767284
GTGCTAG4350.08.749191
TGCACCG1451.6904762E-58.5173115
GTATTAG6500.08.490081
CTTGGAC4700.08.4899383
CTAGCAC4150.08.4704553
GTATTAA6700.08.3786551
ATAGGAC3750.08.3605683
CTTACTC4550.08.3522163
CCGTGTT2409.385985E-108.310889
TAGGACA5150.08.3017334
Produced by FastQC (version 0.11.5)