FastQCFastQC Report
Wed 25 May 2016
SRR1294964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188809
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT115760.27635540317068646No Hit
GGTATCAACGCAGAGTACTTTTTTT76890.1835605299740332No Hit
TATCAACGCAGAGTACTTTTTTTTT64820.15474565682035157No Hit
CCCATGTACTCTGCGTTGATACCAC46080.1100074030589602No Hit
GTACATGGGAAGCAGTGGTATCAAC45250.10802593290837562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC701.0911438E-412.2150153
GCGAAAC600.00588109111.08267211
CATAGCG856.6015363E-410.0582247
TATGCCG900.00111397889.50033955
CCGGTCA1054.4981786E-49.0470799
GGTATCA39000.08.9680631
GTATCAA67350.08.7915591
GTATTAG6650.08.5752161
GTACTAG3600.08.4481751
TATACTG8750.08.3602995
AACCGTG1252.746953E-48.3595017
CTAGGAC4150.08.2414563
TAGGACT5650.08.2392344
CAGGACT7150.08.2380574
AGGACTG7100.08.0284575
TAGGAGT4150.08.0123354
ACCGCAG1200.00169280227.9161948
CTAAGAC4700.07.88344963
ATTAGAC3500.07.87189873
TAGACAG7250.07.86235055
Produced by FastQC (version 0.11.5)