FastQCFastQC Report
Wed 25 May 2016
SRR1294963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4464587
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69180.15495274254931085No Hit
GTACATGGGAAGCAGTGGTATCAAC56610.12679784266719407No Hit
CCCATGTACTCTGCGTTGATACCAC49480.11082772045880168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG1051.376975E-913.5690679
ACGGATC655.464146E-513.1519988
CGTCCGC1352.7284841E-1112.66474510
TCGCGGT1001.4195575E-712.3667051
TCCGTCA4350.012.22816858
TTCGCCC3950.012.02524719
CGTCAGG4200.011.3078110
TAACGCG1354.7493813E-911.2575517
TTTCGCC4500.011.18882318
GCTCCGT4850.011.1632556
CCGTCAG4350.010.917649
AACGCGT1553.6779966E-910.4178928
CTTTCGC4400.010.36287817
CGGCGTC856.6032156E-410.05797514
CGCGTGA1701.5661499E-910.05729810
ACCGTCC951.6477221E-49.9985968
TGTGCGA951.6479084E-49.99848310
CCGTCCA951.6482812E-49.9982599
GCTTTCG4650.09.80584416
GCGGTAC1651.0226358E-89.78858953
Produced by FastQC (version 0.11.5)