FastQCFastQC Report
Wed 25 May 2016
SRR1294963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4464587
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT197020.44129501788183323No Hit
GGTATCAACGCAGAGTACTTTTTTT122790.27503103870525986No Hit
TATCAACGCAGAGTACTTTTTTTTT107590.2409853363816183No Hit
ACGCAGAGTACTTTTTTTTTTTTTT62120.13913940975951414No Hit
CCCATGTACTCTGCGTTGATACCAC55820.12502836208589954No Hit
GTACATGGGAAGCAGTGGTATCAAC55740.124849174178933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA1459.822543E-1111.79220710
TTCGCCC3100.011.64427619
TTTCGCC3200.011.28039318
CCGTCCG600.005881908311.0824919
AACGCGT1502.1391315E-910.7658488
CTTTCGC3550.010.43582617
GCTTTCG3550.010.43582616
CGTCAGG3750.010.13256310
GGTATCA47300.010.0081911
TCGCGGT1002.740667E-49.5057711
CGGAATA900.00111400779.5003414
GGTTGCC7300.09.4992778
TCGCGTC900.00111514949.49927715
CTTAGGC2950.09.3393193
TGCCCTA7250.09.3027411
CTATTCG7050.09.29716515
GTATCAA87100.09.2438451
TAACGCG1752.6773705E-89.2278697
TTCGGAC7000.09.22786918
CCGATCG1252.7590973E-59.11930715
Produced by FastQC (version 0.11.5)