FastQCFastQC Report
Wed 25 May 2016
SRR1294962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3627345
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC95300.2627265947959182No Hit
CCCATGTACTCTGCGTTGATACCAC85230.23496524317372625No Hit
GAGTACATGGGAAGCAGTGGTATCA40560.11181732093307915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG655.4605283E-513.1527855
ACCGGGC604.101442E-412.6640748
TAACCCG802.871198E-511.87404255
CCGCTTG600.00588718411.0810649
ATACCGG600.00588718411.0810646
CGGCCGA750.0026535310.1312596
AACACCG856.6023495E-410.0580135
TACCGTG1356.4680717E-79.8498347
GTACTAG1752.5120244E-99.78591
CCCTATA2600.09.5004142
CACCGTT1106.856788E-59.49805457
TTAGTAC2650.09.3210333
GTCTTAG4050.09.1616961
GTACGGA1151.0936035E-49.1004141
AAGACGG950.00182601758.9992755
ACCGTTC950.00182808188.99815758
AGGACCG1107.153852E-48.6356685
ATACCGT1107.162694E-48.6345956
GTCTTAC3750.08.6260891
TGGACCG1000.00290845688.5493125
Produced by FastQC (version 0.11.5)