FastQCFastQC Report
Wed 25 May 2016
SRR1294962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3627345
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT103930.2865181007045098No Hit
CCCATGTACTCTGCGTTGATACCAC94380.26019030447889574No Hit
GTACATGGGAAGCAGTGGTATCAAC92860.2559999117812064No Hit
GGTATCAACGCAGAGTACTTTTTTT63190.17420454905723057No Hit
TATCAACGCAGAGTACTTTTTTTTT57500.1585181448139066No Hit
GAGTACATGGGAAGCAGTGGTATCA39260.1082334324416343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.005277419514.2514165
GGCGACC604.0975984E-412.66548212
TCGTCCG600.005882315311.0822969
TAGGACG909.512598E-510.5566044
CGGCGTC856.603142E-410.05788114
ATCGTCC900.0011152229.4991118
TAGACTG5250.09.0485185
TATATCG950.00182302979.0008955
GCGTGCG850.00744446278.9403399
GTCCCGC1201.7540541E-48.7132841
TGCACCG1201.7629952E-48.7091995
GACCGTG1107.1547786E-48.6355557
GTCCTAC2502.3283064E-108.3647531
GGGTTAC2053.828609E-88.3462051
TAAGACT4100.08.3422934
GTCTAGG4450.08.3305761
TAGGACC2303.6925485E-98.2616914
GGCGTGC1150.00111217188.2600978
TGCTCGT1150.00111217188.26009710
CACCGTT1150.00111217188.2600977
Produced by FastQC (version 0.11.5)