FastQCFastQC Report
Wed 25 May 2016
SRR1294961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551550
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC67640.19045205614450028No Hit
CCCATGTACTCTGCGTTGATACCAC61370.17279779251312807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT500.001497183113.3035644
AGGACCG802.0005136E-613.0630525
TACGAGT550.003063627312.0941494
CGTGCGG658.044027E-411.68950710
AAGACCG856.5948215E-410.0592495
TAGACAG4950.09.7882935
GTTCTAG4250.09.1799761
GTCCCGG1252.7025546E-59.1351981
GTGTTAG4750.09.01499751
TAGTACG950.0018202169.0024122
GTCTAGG3350.08.8056941
GTCTTGC7650.08.7072961
AACAACG1000.00291298638.5478327
ATACCGT1000.00291298638.5478326
GTCTAAA3800.08.5141641
GTATTAA6350.08.3919131
CTTATAC5100.08.384483
GTATAGG4200.08.3829931
GTTAGAC1606.4327432E-68.314613
GTCTTAC4150.08.2546961
Produced by FastQC (version 0.11.5)