FastQCFastQC Report
Wed 25 May 2016
SRR1294961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551550
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96230.27095211949712095No Hit
CCCATGTACTCTGCGTTGATACCAC68740.19354929537807436No Hit
GTACATGGGAAGCAGTGGTATCAAC68600.19315510129380128No Hit
GGTATCAACGCAGAGTACTTTTTTT62250.17527558389998732No Hit
TATCAACGCAGAGTACTTTTTTTTT54080.15227154341062354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC500.001501063913.2989958
ATCGACC550.003071518412.0899958
CCGTGCG600.00588148711.0824969
CGACCGC600.00588148711.08249610
CTAATAC4300.010.8255243
ACCGTCC803.775884E-410.6866928
ACACCGT1201.5254027E-610.2916146
TGCACGG1151.0243039E-59.9155042
TTAGACT3450.09.91299254
CTAGGAC3100.09.499953
TGCGTTG19900.09.35607712
TTGGACC3000.09.1832864
CTGCGTT20500.09.12857811
TAATACT6700.09.074584
AATACCG850.0074398.9411315
CCGTTCA850.00744329638.9405019
CTCTGCG21650.08.8191969
TAGACTG4850.08.8143875
CTAGTAC2401.0186341E-108.7082883
TCTGCGT21500.08.70399410
Produced by FastQC (version 0.11.5)