FastQCFastQC Report
Wed 25 May 2016
SRR1294960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304852
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA130404.2774854683584165No Hit
GTACATGGGAAGCAGTGGTATCAAC112683.696219805020141No Hit
CCCATGTACTCTGCGTTGATACCAC107453.5246611470484037No Hit
GAGTACATGGGAAGCAGTGGTATCA52861.7339561492133888No Hit
CATGTACTCTGCGTTGATACCACTG45461.491215409444583No Hit
GCGTTGATACCACTGCTTCCCATGT34271.1241520475509428No Hit
ACGCAGAGTACATGGGAAGCAGTGG33021.0831485442116173No Hit
GGTATCAACGCAGAGTACATGGGAA31071.01918307900227No Hit
TATCAACGCAGAGTACATGGGAAGC30501.0004854814795376No Hit
GTATCAACGCAGAGTACATGGGAAG30350.9955650610788186No Hit
ACTCTGCGTTGATACCACTGCTTCC29210.958169866033354No Hit
GTGGTATCAACGCAGAGTACATGGG23060.7564326296038734No Hit
GTATCAACGCAGAGTACTTTTTTTT22650.7429834805085747No Hit
GCTTCCCATGTACTCTGCGTTGATA19110.6268615590516053No Hit
GTACTCTGCGTTGATACCACTGCTT16860.5530552530408198No Hit
GGTATCAACGCAGAGTACTTTTTTT16630.5455106084263839No Hit
GATACCACTGCTTCCCATGTACTCT15650.5133638618083529No Hit
ATACCACTGCTTCCCATGTACTCTG15530.5094275254877777No Hit
GGGAAGCAGTGGTATCAACGCAGAG14380.4717043024155984No Hit
CTGCTTCCCATGTACTCTGCGTTGA13800.45267867686615143No Hit
CATGGGAAGCAGTGGTATCAACGCA13770.45169459278600765No Hit
CAGTGGTATCAACGCAGAGTACATG13770.45169459278600765No Hit
TATCAACGCAGAGTACTTTTTTTTT13500.4428378360647134No Hit
GCAGAGTACATGGGAAGCAGTGGTA13470.44185375198456955No Hit
ACATGGGAAGCAGTGGTATCAACGC12490.4097070053665385No Hit
GTTGATACCACTGCTTCCCATGTAC12220.4008502486452442No Hit
TACCACTGCTTCCCATGTACTCTGC11990.3933056040308084No Hit
GCAGTGGTATCAACGCAGAGTACAT11790.38674504349651634No Hit
CCACTGCTTCCCATGTACTCTGCGT10790.3539422408250561No Hit
CTTCCCATGTACTCTGCGTTGATAC9960.3267159146077441No Hit
AAGCAGTGGTATCAACGCAGAGTAC9080.29784944825685905No Hit
ATGGGAAGCAGTGGTATCAACGCAG8600.2821041029745581No Hit
TGATACCACTGCTTCCCATGTACTC8180.2683269258525448No Hit
CCATGTACTCTGCGTTGATACCACT7980.26176636531825276No Hit
TTCCCATGTACTCTGCGTTGATACC7960.2611103092648236No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7020.23027567475365096No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA6910.2266673664597903No Hit
ATCAACGCAGAGTACATGGGAAGCA6630.21748258171178145No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5660.185663863120465No Hit
AAACAAAAAAAAAAAAAAAAAAAAA5580.1830396389067482No Hit
GTACATGGTAAGCAGTGGTATCAAC5260.17254274205188092No Hit
GGAAGCAGTGGTATCAACGCAGAGT4480.14695655596814192No Hit
ACCATGTACTCTGCGTTGATACCAC4160.13645965911327462No Hit
AAAAACAAAAAAAAAAAAAAAAAAA3950.129571070552268No Hit
CTCTGCGTTGATACCACTGCTTCCC3920.12858698647212416No Hit
AAAAAGTACTCTGCGTTGATACCAC3750.12301051001797594No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3720.12202642593783214No Hit
CTGCGTTGATACCACTGCTTCCCAT3450.11316966921653786No Hit
GTGGTATCAACGCAGAGTACTTTTT3350.10988938894939183No Hit
TCAACGCAGAGTACATGGGAAGCAG3280.10759319276238961No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3280.10759319276238961No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA3170.10398488446852899No Hit
TCCATGTACTCTGCGTTGATACCAC3100.10168868828152677No Hit
TGGGAAGCAGTGGTATCAACGCAGA3080.10103263222809758No Hit
AAAGTACTCTGCGTTGATACCACTG3080.10103263222809758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG250.006021194619.05
GTGTTCA250.00602690818.9968819
GAAACTC350.00216481716.28571519
GTACAAA456.6734187E-414.7972091
GACATAC400.005220488614.2687381
TTGCTCT551.9520223E-413.8181824
GAACAAA2100.013.5892731
ACATACA500.001494725913.2999992
CTTGCTC604.075267E-412.6666673
GACTACA600.005800255611.0979071
CTACAGG600.005857299511.0833343
GTTTCCA600.005864463711.0815149
GCTCTGT700.001487484310.8553616
GAGAAAA1158.580082E-710.7532511
GTAAAAA909.33142E-510.5694351
GTCTTGA750.002608526510.1466571
AAGTACT1106.786254E-59.54
TCTTGCT900.00110811159.52
AGTACTC1201.7509326E-48.7083345
CATGGTA1554.1581206E-68.5806464
Produced by FastQC (version 0.11.5)