##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294960_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	304852
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.980469211289414	31.0	30.0	33.0	16.0	34.0
2	29.26924212404708	31.0	30.0	34.0	16.0	34.0
3	29.513012871819768	31.0	30.0	34.0	18.0	34.0
4	32.54030808392269	37.0	33.0	37.0	17.0	37.0
5	33.37097345597208	37.0	33.0	37.0	27.0	37.0
6	33.27280778869746	37.0	35.0	37.0	23.0	37.0
7	33.52025901092989	37.0	35.0	37.0	26.0	37.0
8	33.565074199939644	37.0	35.0	37.0	25.0	37.0
9	34.61312046501253	39.0	35.0	39.0	22.0	39.0
10	34.95666093711046	39.0	35.0	39.0	25.0	39.0
11	34.836061433088844	39.0	35.0	39.0	24.0	39.0
12	34.95887512629079	39.0	35.0	39.0	25.0	39.0
13	34.55280267146025	39.0	35.0	39.0	22.0	39.0
14	35.67322504034745	40.0	36.0	41.0	22.0	41.0
15	35.65406492330705	40.0	35.0	41.0	22.0	41.0
16	35.57556125595371	40.0	35.0	41.0	22.0	41.0
17	35.57495768438455	40.0	35.0	41.0	22.0	41.0
18	35.453711965150305	40.0	35.0	41.0	21.0	41.0
19	35.45396782701114	40.0	34.0	41.0	21.0	41.0
20	35.491822261294004	40.0	34.0	41.0	22.0	41.0
21	35.425941112408644	40.0	34.0	41.0	22.0	41.0
22	35.50389697295737	40.0	34.0	41.0	22.0	41.0
23	35.48846325430045	40.0	34.0	41.0	22.0	41.0
24	35.63080445593271	40.0	34.0	41.0	24.0	41.0
25	34.61817865718447	39.0	32.0	41.0	20.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.24470971255835394
1101	2	-0.08195096243489886
1101	3	-0.30317148018904305
1101	4	-0.320232453257276
1101	5	-0.32566418482326753
1101	6	-0.06766878057927528
1101	7	-0.0975864412634877
1101	8	-0.0638003555400033
1101	9	0.016207759298339397
1101	10	0.052053698313031305
1101	11	0.10339691118821293
1101	12	0.32500607013046334
1101	13	0.3165904250328353
1101	14	0.1353744395196017
1101	15	0.10023014366805683
1101	16	0.198007417481584
1101	17	0.20721955109723922
1101	18	0.22345127457201386
1101	19	0.47945446360849786
1101	20	0.15633790773244982
1101	21	-0.0451258579390057
1101	22	-0.3448243261751074
1101	23	-0.28887840356481576
1101	24	-0.15446104860067322
1101	25	-0.18312396762295435
1102	1	-0.11224812380385885
1102	2	-0.018275050246398195
1102	3	-0.16389016653701916
1102	4	-0.3020766959710812
1102	5	-0.34932199154743415
1102	6	0.13971925472637992
1102	7	-0.10046319837365303
1102	8	0.2337025991711883
1102	9	-0.004170946663890618
1102	10	-0.1456982065326926
1102	11	0.11152928224964853
1102	12	0.5406823846626168
1102	13	0.29602060583601997
1102	14	0.29991287591158766
1102	15	0.32089049018652815
1102	16	0.47943171400886087
1102	17	0.07737822537490757
1102	18	0.4382021987050919
1102	19	-0.013557330089795983
1102	20	0.05074784562064849
1102	21	-0.20505745951820842
1102	22	-0.18777954029737032
1102	23	0.16546816519822727
1102	24	0.08905241733842217
1102	25	-0.09618756306640819
1103	1	0.10598153168127666
1103	2	0.12687569477885674
1103	3	-0.28087442240811455
1103	4	0.10355343827055208
1103	5	-0.09078293703879581
1103	6	-0.022968319749786303
1103	7	0.5180817615014846
1103	8	0.29490223219484335
1103	9	0.6343573516414764
1103	10	0.095478015930901
1103	11	0.40903321037289686
1103	12	0.680518299836244
1103	13	0.35540644771982954
1103	14	0.1274339928694843
1103	15	0.15322553537313155
1103	16	0.20871284109023946
1103	17	0.38226386162541814
1103	18	0.48417442239193775
1103	19	0.2873949102586195
1103	20	0.4420521934467345
1103	21	0.39602976063596884
1103	22	0.3819037844239688
1103	23	0.3592960026741068
1103	24	0.285595668875402
1103	25	0.048035195794277286
1104	1	-0.40250192035056287
1104	2	-0.3523555578394948
1104	3	-0.2962284232459851
1104	4	-0.1521755102003297
1104	5	-0.24069914985823004
1104	6	-0.07935709226759258
1104	7	-0.015118908795955122
1104	8	-0.22893522067487027
1104	9	-0.30899204090146526
1104	10	0.06186888312821992
1104	11	-0.07509959230829111
1104	12	0.36184423196862525
1104	13	0.29199002543244035
1104	14	-0.1345056803605118
1104	15	-0.16145816802025337
1104	16	-0.039904670430502165
1104	17	0.1561955750732622
1104	18	-0.13896131284057134
1104	19	0.02595796011199525
1104	20	-0.3193114429168986
1104	21	-0.19112985394300352
1104	22	-0.292876274227055
1104	23	-0.2757165654029805
1104	24	-0.37299251713213977
1104	25	0.0024654429664607846
1105	1	-0.2220879407374987
1105	2	-0.4164443866308396
1105	3	-0.31949657385967356
1105	4	-0.32724832068504384
1105	5	-0.44085517232547033
1105	6	-0.2538734226191579
1105	7	-0.2881062189686219
1105	8	-0.29718984934140025
1105	9	-0.36115035169821397
1105	10	-0.761258847264962
1105	11	-0.31638833788765197
1105	12	-0.4227043430261759
1105	13	-0.6833844284889281
1105	14	-0.9334967350723957
1105	15	-0.573846351918732
1105	16	-0.8569527253504106
1105	17	-0.5336057563184582
1105	18	-0.5091662223475382
1105	19	-0.9952506939341887
1105	20	-0.5190462047225992
1105	21	-0.5620368645525318
1105	22	-0.5928138652401671
1105	23	-0.5484906448704265
1105	24	-0.6592313355219872
1105	25	-0.42799534338677603
1106	1	-0.33378534281045447
1106	2	-0.2042004776255908
1106	3	-0.333501150518714
1106	4	-0.08049101822172133
1106	5	-0.7332659947780158
1106	6	-0.09187046125154552
1106	7	-0.430359550507184
1106	8	-0.30925607628984153
1106	9	-0.21008829758595482
1106	10	-0.5911525072461146
1106	11	-0.600313496051605
1106	12	-0.274011383004634
1106	13	-0.25434687296328207
1106	14	-0.45402439693805263
1106	15	-0.0698538899453851
1106	16	0.02068356486361722
1106	17	-0.05607715219946385
1106	18	-0.09166184759993001
1106	19	-0.2830794730430881
1106	20	-0.3461831006709133
1106	21	-0.6486164849590494
1106	22	-0.19490835978855614
1106	23	-0.16515506807677838
1106	24	-0.37165561873641906
1106	25	-0.3800211880495823
1107	1	0.7413085245237134
1107	2	0.6924703609225951
1107	3	-0.022437320731476973
1107	4	0.19265040856175375
1107	5	0.2330919526562738
1107	6	0.4411458090863789
1107	7	0.2932950177030733
1107	8	0.40781526288412095
1107	9	0.6589479977832582
1107	10	0.5535033125286049
1107	11	0.870500020844517
1107	12	0.6607320462819715
1107	13	0.7502588842332116
1107	14	0.7710068921733324
1107	15	0.9367043078017971
1107	16	0.8547761806333298
1107	17	0.5890292307792606
1107	18	0.8371992287455043
1107	19	0.6040043817754395
1107	20	0.6271585764254581
1107	21	0.5575161808732432
1107	22	0.5682455826394772
1107	23	0.8902892373049482
1107	24	0.23735564534368336
1107	25	0.6793029671444089
1108	1	0.5855566312673126
1108	2	0.3427137796027253
1108	3	0.572685290159253
1108	4	0.7378090814791776
1108	5	0.12228236015717897
1108	6	0.6116290402439688
1108	7	0.6476314764120588
1108	8	0.30812179011095253
1108	9	0.4772945538597071
1108	10	0.8454975426043347
1108	11	0.24423784991050468
1108	12	0.7955499330472122
1108	13	0.549501581672807
1108	14	0.9785528712573566
1108	15	1.0460533882201162
1108	16	0.4011970244847518
1108	17	0.6090075935632484
1108	18	0.9754069460192127
1108	19	0.7940242158901114
1108	20	0.901797956158596
1108	21	0.7140704542234033
1108	22	0.599304003122711
1108	23	0.8058319708065724
1108	24	0.6508518588308831
1108	25	0.6102081240142212
1109	1	0.21604094208231928
1109	2	0.07965240364912063
1109	3	0.06397339123281398
1109	4	-0.24460746724532356
1109	5	-0.05951973863808746
1109	6	0.09474029710474952
1109	7	-0.2472518471407099
1109	8	-0.008846348243835678
1109	9	-0.48926884149394567
1109	10	-0.34339403114691436
1109	11	-0.6788998144893696
1109	12	-0.13629963027910463
1109	13	-0.3412954772522525
1109	14	-0.4752296203716213
1109	15	-0.44734041204913666
1109	16	-0.003550291611766454
1109	17	0.031010424175278217
1109	18	0.0652184686813726
1109	19	0.006730039356575901
1109	20	-0.38890503669583154
1109	21	-0.0964899724395778
1109	22	-0.24962502445849566
1109	23	-0.22798003513080545
1109	24	-0.5084952018201605
1109	25	-0.596530113411994
1110	1	-0.7227498542348592
1110	2	-0.8025621694097396
1110	3	-0.6892036298575555
1110	4	-1.4453573283821513
1110	5	-0.6906991498582329
1110	6	-0.9942331253254437
1110	7	-0.8353668426802514
1110	8	-0.861579848774042
1110	9	-0.5707689004055965
1110	10	-0.7321393813345907
1110	11	-0.5244797575975468
1110	12	-0.8486929581140217
1110	13	-0.884662867129542
1110	14	-1.0353321266415065
1110	15	-1.2527804820698378
1110	16	-0.9419707861329769
1110	17	-1.086573019968057
1110	18	-0.8618951971380895
1110	19	-0.7475957588962672
1110	20	-0.7438982197764048
1110	21	-0.6547662175793647
1110	22	-0.34783495191300773
1110	23	-0.5912124331715773
1110	24	-0.5494387981238731
1110	25	-0.5382370363723865
1111	1	-0.3247320339869262
1111	2	-0.28758283056676603
1111	3	0.11009259948128758
1111	4	-0.2456059079276045
1111	5	-0.1386750021309595
1111	6	-0.16856163772213506
1111	7	0.0026367730222247587
1111	8	-0.07101618658396092
1111	9	-0.34407726817418904
1111	10	-0.0671083895990563
1111	11	-0.06604419458101773
1111	12	-0.4109185521222827
1111	13	-0.1538551450221064
1111	14	0.02529545600312133
1111	15	-0.13386583847479727
1111	16	0.31204845456949926
1111	17	-0.5261552771994644
1111	18	-0.23718574465875264
1111	19	-0.3618616421607328
1111	20	-0.16927593155326548
1111	21	-0.3921596834884582
1111	22	-0.39493593331796717
1111	23	-0.022094406312071158
1111	24	-0.007616096677594442
1111	25	-0.13684563657899673
1112	1	0.11878235093371003
1112	2	-0.028654259138193083
1112	3	0.25586392884636666
1112	4	0.14573213770731286
1112	5	0.11575828159919865
1112	6	-0.31290687581737586
1112	7	-0.2836975523745977
1112	8	-0.30635224809189765
1112	9	-0.6219790538884737
1112	10	-0.2858583895990563
1112	11	-0.21903898624768203
1112	12	-0.2781990580746623
1112	13	-0.0689407104982962
1112	14	-0.008062678917511334
1112	15	-0.36405557061765137
1112	16	-0.12244435297018441
1112	17	-0.24171207283438179
1112	18	-0.20847790735716387
1112	19	-0.0215528623988277
1112	20	-0.22263034623580324
1112	21	0.25234056452741527
1112	22	0.27303281668203283
1112	23	0.04369180400539108
1112	24	0.19547790133827903
1112	25	-0.07946096395994573
1113	1	0.24489818434320654
1113	2	0.13168300436605662
1113	3	0.2787061431473141
1113	4	0.409598176765293
1113	5	0.09450413054658213
1113	6	-0.033064996647276246
1113	7	0.10383938818537075
1113	8	-0.504681163791254
1113	9	-0.1699979735484689
1113	10	-0.2719428425741057
1113	11	-0.3861055249552976
1113	12	-0.2583311300973321
1113	13	-0.21963534535800022
1113	14	-0.48134741827711025
1113	15	-0.12159601481836546
1113	16	-0.07570121553606413
1113	17	-0.31219999289044154
1113	18	-0.2597910109735295
1113	19	-0.1770345368344337
1113	20	-0.1510380176856998
1113	21	0.07630165888198093
1113	22	-0.06645854608188273
1113	23	0.016915610482556076
1113	24	0.12476529127825842
1113	25	0.14835185022522523
1114	1	0.1903011099524683
1114	2	0.23893232094838623
1114	3	0.2882412737237132
1114	4	0.2250972170723955
1114	5	0.4835432743841892
1114	6	0.43087018045968506
1114	7	0.1913204851434358
1114	8	0.09227690053725013
1114	9	-0.17717385908328254
1114	10	-0.2157826320232985
1114	11	-0.14328141049011123
1114	12	0.02320786833226407
1114	13	-0.21558573214331744
1114	14	0.06814583479099667
1114	15	-0.18683695589903948
1114	16	0.2797922992664681
1114	17	-0.044562000684315706
1114	18	-0.4226734340526903
1114	19	0.4259579601119938
1114	20	0.025385526780070222
1114	21	0.2334913581782061
1114	22	0.20863887728809516
1114	23	0.0587531315667178
1114	24	0.5251135434739211
1114	25	-0.011549708548692195
1115	1	0.2159371877111873
1115	2	-0.029328113585634696
1115	3	0.18310262306619407
1115	4	-0.17254429236156454
1115	5	0.3442922738124352
1115	6	-0.0040569207410001695
1115	7	-0.06631959490230344
1115	8	-0.03493865120660189
1115	9	-0.16786854175909127
1115	10	0.11319821417453113
1115	11	0.19526918985294373
1115	12	0.2815269077833804
1115	13	-0.12091735021078875
1115	14	0.0707258805314197
1115	15	0.08837030539312707
1115	16	-0.19367825533615957
1115	17	-0.2092846993692774
1115	18	-0.3797417588096934
1115	19	-0.05508835206639162
1115	20	0.25148615571088584
1115	21	0.30055110660588014
1115	22	0.27539130724807137
1115	23	-0.039738863859241746
1115	24	0.17151863209598872
1115	25	0.2615992337040254
1116	1	0.41649928717056994
1116	2	-0.061657783128435284
1116	3	0.346802454794382
1116	4	0.13947103111414094
1116	5	0.048821291749497675
1116	6	0.06418266967824593
1116	7	0.029602498828673163
1116	8	0.1031158402186989
1116	9	-0.12872207367703226
1116	10	-0.18007091331821812
1116	11	-0.12471577309145232
1116	12	-0.12091840387750352
1116	13	-0.06299962438643547
1116	14	-0.6988061806192718
1116	15	-0.26165654649187786
1116	16	-0.15669783931095793
1116	17	-0.037633129429067935
1116	18	-0.16247778687886694
1116	19	0.2627269785698161
1116	20	0.047365286425858244
1116	21	-0.2983318549780236
1116	22	0.15704609941447956
1116	23	0.27051855621164833
1116	24	0.33005216114973024
1116	25	0.3216865918365599
1201	1	-0.43578114112978383
1201	2	0.13429216943323397
1201	3	-0.17171543623299712
1201	4	-0.4780277829276045
1201	5	-0.4894004485595289
1201	6	-0.34043663772213506
1201	7	-0.35115786983492114
1201	8	-0.617612168726815
1201	9	-0.10635405388847374
1201	10	-0.4644298181704869
1201	11	-0.43406874815244834
1201	12	0.12953903716343262
1201	13	-0.4833752343078217
1201	14	-0.8396599011397328
1201	15	-0.5515555706176514
1201	16	-0.7657975275733548
1201	17	-0.5197378664851797
1201	18	-0.5189937803730373
1201	19	-1.031225481446448
1201	20	-0.6360728065532655
1201	21	-0.6296038799170276
1201	22	-0.5327707547465366
1201	23	-0.6755542277406406
1201	24	-0.871734400249025
1201	25	-0.39270501157899673
1202	1	-0.1753311090990337
1202	2	-0.22452229245017463
1202	3	-0.42776124020481276
1202	4	-0.1678400026585436
1202	5	-0.23187322242243624
1202	6	0.17518836227786494
1202	7	0.05122842100428926
1202	8	-0.32163803355705767
1202	9	-0.11261005965400983
1202	10	-0.14123955551833944
1202	11	-0.15831402361688873
1202	12	-0.14780721523887763
1202	13	-0.08586362316111718
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2209	23	0.6643191419894166
2209	24	0.755583047430683
2209	25	0.8315187145356546
2210	1	0.628759675196747
2210	2	0.5356314551475201
2210	3	0.6979274209098598
2210	4	0.7909135436030112
2210	5	0.8799873065425103
2210	6	0.4731475459513348
2210	7	0.6146584566956932
2210	8	0.6699516067833855
2210	9	0.8132250277441813
2210	10	1.1110803859111513
2210	11	0.8073215579700062
2210	12	0.6593859759389389
2210	13	0.8103109901819749
2210	14	1.1845110172276136
2210	15	1.0451281028517343
2210	16	1.1978504316103127
2210	17	1.0692289702495117
2210	18	1.0032000971779809
2210	19	0.803787273655594
2210	20	0.6828685199773474
2210	21	0.5288144874299121
2210	22	0.8592573064779501
2210	23	0.7420478130756862
2210	24	0.928584477301996
2210	25	1.1974097843393707
2211	1	0.10626835501722454
2211	2	0.2225312773170529
2211	3	0.28127063371365324
2211	4	0.5222963871968744
2211	5	0.3413642224541036
2211	6	0.042408279290313544
2211	7	0.26732167966122944
2211	8	0.48389012825006716
2211	9	0.34890120692954696
2211	10	0.28156899629306054
2211	11	0.15146585469284446
2211	12	0.42173672536734585
2211	13	-0.12638983122542413
2211	14	0.26845546637656525
2211	15	0.13935284669117465
2211	16	0.11299989388485443
2211	17	0.31222160298725754
2211	18	0.468743326918009
2211	19	-0.03056787919392434
2211	20	-0.24466979825451318
2211	21	0.049967845038516145
2211	22	0.11587514033348612
2211	23	0.030988451571744235
2211	24	-0.13396950694730236
2211	25	-0.07936216925534723
2212	1	-0.13307376785789415
2212	2	0.02088491136871795
2212	3	0.16238594625547975
2212	4	-0.37772536357276465
2212	5	0.42477005835291237
2212	6	-0.10706970223826318
2212	7	-0.2183813721390635
2212	8	-0.24484431158396092
2212	9	-0.019732510109669477
2212	10	-0.0015841960506719488
2212	11	-0.18946909377456933
2212	12	-0.3186176448642186
2212	13	-0.21112581034469002
2212	14	0.044196665680537706
2212	15	-0.29925142315221365
2212	16	-0.4237756381724367
2212	17	-0.3009158618768808
2212	18	0.004623731147695764
2212	19	-0.08962121877363671
2212	20	0.018665096672542347
2212	21	-0.21140111494007385
2212	22	-0.3096284736405508
2212	23	0.09748724691373667
2212	24	0.1631878347740212
2212	25	-0.05449936641770847
2213	1	0.4468967354683251
2213	2	0.3503062744916008
2213	3	0.3058762813879028
2213	4	0.2839493571502132
2213	5	0.26685302559277346
2213	6	0.2646825257019927
2213	7	-0.017152867055592935
2213	8	0.19338526040047554
2213	9	0.11169243026939313
2213	10	0.16647624075113754
2213	11	0.136830015049334
2213	12	0.11394009330573596
2213	13	-0.031188255430663503
2213	14	0.2829863606723819
2213	15	0.14970207251742096
2213	16	0.17196303384965717
2213	17	0.2513936649211601
2213	18	-0.170293113335795
2213	19	0.23856850132175822
2213	20	0.4887448004506254
2213	21	0.3661923957916997
2213	22	0.5069822330244449
2213	23	0.7443665615867587
2213	24	0.8672091100344659
2213	25	0.7912079261641907
2214	1	0.655121558532759
2214	2	0.3172400040789043
2214	3	0.6213807392450654
2214	4	0.7851365871511362
2214	5	0.6154926897981738
2214	6	0.3031411181833761
2214	7	0.3773291942820691
2214	8	0.3368486018018686
2214	9	0.3202312948179369
2214	10	0.19445657103086234
2214	11	0.19467492451347113
2214	12	0.22184781106669504
2214	13	0.30854335399364174
2214	14	0.4132752788377658
2214	15	0.30411090857244716
2214	16	0.44789060516004753
2214	17	0.36691497378478743
2214	18	0.3774488517979435
2214	19	0.4270316895321784
2214	20	0.7294537682498827
2214	21	0.8488128804879196
2214	22	0.966930454477307
2214	23	0.9879019027430473
2214	24	0.9937218708420872
2214	25	1.099297941176907
2215	1	0.5831055723457759
2215	2	0.578083521040039
2215	3	0.6533419496703239
2215	4	0.8926302983578367
2215	5	0.5725763270278321
2215	6	1.2328443169092438
2215	7	0.6889109932490669
2215	8	0.5956566335956239
2215	9	0.6932720938295915
2215	10	0.4291699657884678
2215	11	0.5111910074511172
2215	12	0.31423725482478915
2215	13	0.5738039109797839
2215	14	0.5259600353982066
2215	15	0.8404407836896652
2215	16	0.9414752917623161
2215	17	0.6706525264394756
2215	18	0.7510068947552355
2215	19	0.8248667073194156
2215	20	0.623526673597965
2215	21	0.6461016291296886
2215	22	0.6607927410676666
2215	23	0.5736862529506865
2215	24	0.772367510363992
2215	25	0.3993850167763924
2216	1	0.2877291193279916
2216	2	0.19687849540008528
2216	3	-0.13887868274707316
2216	4	-0.2594792101835921
2216	5	0.18423806762042005
2216	6	-0.07619285319174907
2216	7	-0.19840201611221175
2216	8	-0.32150177014749914
2216	9	-0.09248023778744852
2216	10	-0.120572938402006
2216	11	-0.08943493468230912
2216	12	-0.26718222040036466
2216	13	-0.2711901185488088
2216	14	-0.5124146038495496
2216	15	-0.2670416269186262
2216	16	-0.4873264015538865
2216	17	-0.06992613930812297
2216	18	-0.2308623675869299
2216	19	-0.19772360693973923
2216	20	0.44941867595133544
2216	21	0.27228049261651677
2216	22	-0.139720406154062
2216	23	0.009018910907080624
2216	24	-0.027445890646284
2216	25	-0.2274483357042243
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81.0
3	40.0
4	12.0
5	21.0
6	17.0
7	30.0
8	18.0
9	107.0
10	235.0
11	463.0
12	887.0
13	1629.0
14	2408.0
15	3562.0
16	4357.0
17	4789.0
18	4859.0
19	4544.0
20	4067.0
21	3742.0
22	3465.0
23	3120.0
24	2840.0
25	2682.0
26	2667.0
27	2507.0
28	2620.0
29	2817.0
30	3261.0
31	4066.0
32	5242.0
33	6863.0
34	9535.0
35	14272.0
36	23947.0
37	49791.0
38	129289.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41795133744822	25.089759972144023	11.24455116729025	21.247737523117504
2	12.842994685408343	30.044676709351908	32.398656744344834	24.713671860894912
3	19.51348388727162	36.76045382790116	24.8092367171321	18.91682556769512
4	12.381937263012755	28.576352981730434	27.710313356642207	31.331396398614597
5	10.074544804020364	46.44197092391572	30.21116104106693	13.272323230996982
6	27.220387718526588	39.68698917460463	16.583074569839884	16.50954853702889
7	19.876190257368027	42.60538762755981	19.852885666363598	17.665536448708565
8	24.10639548052272	40.92411624326658	18.884442795852113	16.08504548035859
9	24.39726508519529	26.37098600703095	17.8130528831162	31.41869602465756
10	15.902682724279755	39.30438892773089	26.603053309831186	18.189875038158167
11	23.90368152933144	36.152906885142585	16.72870384958773	23.21470773593824
12	21.2255495704865	40.06702839680023	23.976930677144367	14.730491355568903
13	26.111975525049402	31.36608039706143	20.48464078676987	22.037303291119294
14	19.57100421774736	36.35435642426928	22.400013129174667	21.67462622880869
15	28.90635769849345	31.873174254111003	20.32953687596416	18.890931171431387
16	16.59937765873641	36.36265164644714	27.98664723491413	19.051323459902317
17	21.43187749273721	37.23636483004251	23.87086185106767	17.46089582615261
18	21.653542014751693	31.791966334365142	27.351687056653194	19.202804594229967
19	20.079369508060044	37.00496633853163	27.997282135952283	14.91838201745604
20	22.168893032661757	32.177198816836125	25.80997810293263	19.84393004756949
21	19.555454885663075	34.32799564055596	27.78587513869466	18.330674335086304
22	21.26138310933694	33.414965629082985	21.84637808665279	23.477273174927284
23	17.78735669918977	33.73100812858662	26.371945214114064	22.109689958109545
24	20.570361055752368	37.66477662609701	23.63984385003562	18.125018468115
25	20.717322433971866	30.75195745309499	22.292148848508724	26.238571264424422
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13957.0
1	13957.0
2	9482.5
3	5008.0
4	5008.0
5	5008.0
6	4964.5
7	4921.0
8	4921.0
9	4921.0
10	4830.5
11	4740.0
12	4740.0
13	4740.0
14	4858.0
15	4976.0
16	4976.0
17	4976.0
18	5836.0
19	6696.0
20	6696.0
21	6696.0
22	7947.0
23	9198.0
24	9198.0
25	9198.0
26	11225.0
27	13252.0
28	13252.0
29	13252.0
30	14338.5
31	15425.0
32	15425.0
33	15425.0
34	16037.0
35	16649.0
36	16649.0
37	16649.0
38	17131.5
39	17614.0
40	17614.0
41	17614.0
42	19823.0
43	22032.0
44	22032.0
45	22032.0
46	46327.0
47	70622.0
48	70622.0
49	70622.0
50	60876.5
51	51131.0
52	51131.0
53	51131.0
54	37140.5
55	23150.0
56	23150.0
57	23150.0
58	17474.5
59	11799.0
60	11799.0
61	11799.0
62	9546.5
63	7294.0
64	7294.0
65	7294.0
66	5510.5
67	3727.0
68	3727.0
69	3727.0
70	2666.0
71	1605.0
72	1605.0
73	1605.0
74	1159.0
75	713.0
76	713.0
77	713.0
78	484.5
79	256.0
80	256.0
81	256.0
82	162.0
83	68.0
84	68.0
85	68.0
86	41.0
87	14.0
88	14.0
89	14.0
90	9.5
91	5.0
92	5.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14072402346056448
2	0.07183813785049795
3	0.05084434414076339
4	0.08102292259850682
5	0.06658968942306431
6	0.0649495492894913
7	0.06265335310248908
8	0.07052602574363953
9	0.0652775773162059
10	0.0652775773162059
11	0.06429349323606208
12	0.06724574547649351
13	0.07019799771692493
14	0.061341240995630666
15	0.059701100862057654
16	0.06429349323606208
17	0.07118208179706874
18	0.06855785758335192
19	0.0659336333696351
20	0.0803668665450776
21	0.07413433403750017
22	0.076758558251217
23	0.08135095062522142
24	0.08955165129308648
25	0.08102292259850682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	304852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.84723982038056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.58587899945137	33.7229660462621
2	11.328340514245456	9.481195645331255
3	3.850258326301708	4.833680506534939
4	1.5628303084069755	2.6160053885786403
5	0.8301549229080935	1.7369846073502262
6	0.4331252165573248	1.087505688571718
7	0.269370341357644	0.7890683692703772
8	0.18635591355510964	0.6238784485189424
9	0.1432883261692323	0.5396598853798298
>10	0.6506667176473935	4.9840466633918945
>50	0.07125229225183037	2.098011034023698
>100	0.05637544002342624	4.801211257465133
>500	0.009395906670571039	2.910281743588706
>1k	0.019574805563689665	16.55800243531994
>5k	7.829922225475865E-4	1.7320141068013848
>10k+	0.0023489766676427597	11.485488173611227
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	13040	4.2774854683584165	No Hit
GTACATGGGAAGCAGTGGTATCAAC	11268	3.696219805020141	No Hit
CCCATGTACTCTGCGTTGATACCAC	10745	3.5246611470484037	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5286	1.7339561492133888	No Hit
CATGTACTCTGCGTTGATACCACTG	4546	1.491215409444583	No Hit
GCGTTGATACCACTGCTTCCCATGT	3427	1.1241520475509428	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3302	1.0831485442116173	No Hit
GGTATCAACGCAGAGTACATGGGAA	3107	1.01918307900227	No Hit
TATCAACGCAGAGTACATGGGAAGC	3050	1.0004854814795376	No Hit
GTATCAACGCAGAGTACATGGGAAG	3035	0.9955650610788186	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2921	0.958169866033354	No Hit
GTGGTATCAACGCAGAGTACATGGG	2306	0.7564326296038734	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2265	0.7429834805085747	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1911	0.6268615590516053	No Hit
GTACTCTGCGTTGATACCACTGCTT	1686	0.5530552530408198	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1663	0.5455106084263839	No Hit
GATACCACTGCTTCCCATGTACTCT	1565	0.5133638618083529	No Hit
ATACCACTGCTTCCCATGTACTCTG	1553	0.5094275254877777	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	1438	0.4717043024155984	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	1380	0.45267867686615143	No Hit
CATGGGAAGCAGTGGTATCAACGCA	1377	0.45169459278600765	No Hit
CAGTGGTATCAACGCAGAGTACATG	1377	0.45169459278600765	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1350	0.4428378360647134	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	1347	0.44185375198456955	No Hit
ACATGGGAAGCAGTGGTATCAACGC	1249	0.4097070053665385	No Hit
GTTGATACCACTGCTTCCCATGTAC	1222	0.4008502486452442	No Hit
TACCACTGCTTCCCATGTACTCTGC	1199	0.3933056040308084	No Hit
GCAGTGGTATCAACGCAGAGTACAT	1179	0.38674504349651634	No Hit
CCACTGCTTCCCATGTACTCTGCGT	1079	0.3539422408250561	No Hit
CTTCCCATGTACTCTGCGTTGATAC	996	0.3267159146077441	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	908	0.29784944825685905	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	860	0.2821041029745581	No Hit
TGATACCACTGCTTCCCATGTACTC	818	0.2683269258525448	No Hit
CCATGTACTCTGCGTTGATACCACT	798	0.26176636531825276	No Hit
TTCCCATGTACTCTGCGTTGATACC	796	0.2611103092648236	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	702	0.23027567475365096	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	691	0.2266673664597903	No Hit
ATCAACGCAGAGTACATGGGAAGCA	663	0.21748258171178145	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	566	0.185663863120465	No Hit
AAACAAAAAAAAAAAAAAAAAAAAA	558	0.1830396389067482	No Hit
GTACATGGTAAGCAGTGGTATCAAC	526	0.17254274205188092	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	448	0.14695655596814192	No Hit
ACCATGTACTCTGCGTTGATACCAC	416	0.13645965911327462	No Hit
AAAAACAAAAAAAAAAAAAAAAAAA	395	0.129571070552268	No Hit
CTCTGCGTTGATACCACTGCTTCCC	392	0.12858698647212416	No Hit
AAAAAGTACTCTGCGTTGATACCAC	375	0.12301051001797594	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	372	0.12202642593783214	No Hit
CTGCGTTGATACCACTGCTTCCCAT	345	0.11316966921653786	No Hit
GTGGTATCAACGCAGAGTACTTTTT	335	0.10988938894939183	No Hit
TCAACGCAGAGTACATGGGAAGCAG	328	0.10759319276238961	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	328	0.10759319276238961	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA	317	0.10398488446852899	No Hit
TCCATGTACTCTGCGTTGATACCAC	310	0.10168868828152677	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	308	0.10103263222809758	No Hit
AAAGTACTCTGCGTTGATACCACTG	308	0.10103263222809758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.280280267146025E-4	0.0
4	0.0	0.0	0.0	3.280280267146025E-4	0.0
5	0.0	0.0	0.0	3.280280267146025E-4	0.0
6	0.0	0.0	0.0	3.280280267146025E-4	0.0
7	0.0	0.0	0.0	3.280280267146025E-4	0.0
8	0.0	0.0	0.0	3.280280267146025E-4	0.0
9	0.0	0.0	0.0	3.280280267146025E-4	0.0
10	0.0	0.0	0.0	6.56056053429205E-4	0.0
11	0.0	0.0	0.0	6.56056053429205E-4	0.0
12	0.0	0.0	0.0	6.56056053429205E-4	0.0
13	0.0	0.0	0.0	6.56056053429205E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	25	0.0060211946	19.0	5
GTGTTCA	25	0.006026908	18.996881	9
GAAACTC	35	0.002164817	16.285715	19
GTACAAA	45	6.6734187E-4	14.797209	1
GACATAC	40	0.0052204886	14.268738	1
TTGCTCT	55	1.9520223E-4	13.818182	4
GAACAAA	210	0.0	13.589273	1
ACATACA	50	0.0014947259	13.299999	2
CTTGCTC	60	4.075267E-4	12.666667	3
GACTACA	60	0.0058002556	11.097907	1
CTACAGG	60	0.0058572995	11.083334	3
GTTTCCA	60	0.0058644637	11.081514	9
GCTCTGT	70	0.0014874843	10.855361	6
GAGAAAA	115	8.580082E-7	10.753251	1
GTAAAAA	90	9.33142E-5	10.569435	1
GTCTTGA	75	0.0026085265	10.146657	1
AAGTACT	110	6.786254E-5	9.5	4
TCTTGCT	90	0.0011081115	9.5	2
AGTACTC	120	1.7509326E-4	8.708334	5
CATGGTA	155	4.1581206E-6	8.580646	4
>>END_MODULE