FastQCFastQC Report
Wed 25 May 2016
SRR1294960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304852
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC118623.8910684528886152No Hit
GTACATGGGAAGCAGTGGTATCAAC111773.666369254589112No Hit
GTATCAACGCAGAGTACTTTTTTTT91533.0024405285187563No Hit
GGTATCAACGCAGAGTACTTTTTTT69322.2738902811856243No Hit
TATCAACGCAGAGTACTTTTTTTTT58471.917979872200281No Hit
GAGTACATGGGAAGCAGTGGTATCA53771.7638066996444175No Hit
CATGTACTCTGCGTTGATACCACTG46741.5332029968640521No Hit
ACGCAGAGTACATGGGAAGCAGTGG37441.228136932019472No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36221.1881175127602903No Hit
GCGTTGATACCACTGCTTCCCATGT35971.1799168120924253No Hit
TATCAACGCAGAGTACATGGGAAGC32271.0585464422080222No Hit
GGTATCAACGCAGAGTACATGGGAA30210.9909726687048142No Hit
ACTCTGCGTTGATACCACTGCTTCC29750.9758833794759425No Hit
GTATCAACGCAGAGTACATGGGAAG29350.9627622584073583No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28920.9486570532586305No Hit
GTGGTATCAACGCAGAGTACATGGG23870.7830028997677562No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21940.7196934906118378No Hit
GCTTCCCATGTACTCTGCGTTGATA19610.6432629603873354No Hit
ATACCACTGCTTCCCATGTACTCTG16280.5340296274913728No Hit
GATACCACTGCTTCCCATGTACTCT16100.5281251230105101No Hit
CATGGGAAGCAGTGGTATCAACGCA15690.5146759739152114No Hit
CAGTGGTATCAACGCAGAGTACATG15570.5107396375946361No Hit
GTACTCTGCGTTGATACCACTGCTT15550.5100835815412069No Hit
ACATGGGAAGCAGTGGTATCAACGC14610.47924894703003423No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14600.4789209190033197No Hit
GGGAAGCAGTGGTATCAACGCAGAG14190.46547176990802097No Hit
CTGCTTCCCATGTACTCTGCGTTGA13640.4474302284387178No Hit
GCAGAGTACATGGGAAGCAGTGGTA13110.43004474302284385No Hit
GCAGTGGTATCAACGCAGAGTACAT12670.41561150984740136No Hit
GTGGTATCAACGCAGAGTACTTTTT12590.4129872856336846No Hit
TACCACTGCTTCCCATGTACTCTGC12130.3978979964048128No Hit
CCACTGCTTCCCATGTACTCTGCGT11570.3795284269087951No Hit
GTTGATACCACTGCTTCCCATGTAC11390.37362392242793224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA11250.3690315300539278No Hit
ATCAACGCAGAGTACTTTTTTTTTT11050.36247096951963576No Hit
CTTCCCATGTACTCTGCGTTGATAC11010.3611588574127773No Hit
ATGGGAAGCAGTGGTATCAACGCAG9750.3198273260467374No Hit
AAGCAGTGGTATCAACGCAGAGTAC9630.3158909897261622No Hit
CCATGTACTCTGCGTTGATACCACT9240.3030978966842927No Hit
TTCCCATGTACTCTGCGTTGATACC8430.2765276265204099No Hit
TGATACCACTGCTTCCCATGTACTC8080.2650466455853988No Hit
ATCAACGCAGAGTACATGGGAAGCA7520.24667707608938108No Hit
GTACATGGTAAGCAGTGGTATCAAC5910.19386456378833009No Hit
GGAAGCAGTGGTATCAACGCAGAGT5420.17779119047931455No Hit
CAACGCAGAGTACTTTTTTTTTTTT4800.15745345282300918No Hit
AACGCAGAGTACTTTTTTTTTTTTT4760.1561413407161508No Hit
ACCATGTACTCTGCGTTGATACCAC4440.14564444386128353No Hit
CTGCGTTGATACCACTGCTTCCCAT3830.12563473423169275No Hit
TGGGAAGCAGTGGTATCAACGCAGA3760.12333853804469054No Hit
TCAACGCAGAGTACTTTTTTTTTTT3730.12235445396454672No Hit
CTCTGCGTTGATACCACTGCTTCCC3670.12038628580425911No Hit
AAAAAGTACTCTGCGTTGATACCAC3640.11940220172411531No Hit
TCAACGCAGAGTACATGGGAAGCAG3580.1174340335638277No Hit
AGTGGTATCAACGCAGAGTACATGG3230.1059530526288166No Hit
CCCCATGTACTCTGCGTTGATACCA3220.105625024602102No Hit
GTACATGGAAAGCAGTGGTATCAAC3190.1046409405219582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA250.006023608618.99868816
GTGAGCT550.003056552512.0920574
GAACAAA1104.890808E-711.2320241
CCCAGCT1105.9976246E-610.3629219
CCAGCTA950.00181586528.99937810
TTTTGTG1107.1058975E-48.63576719
TCCCAGC1401.0310539E-48.1422958
CCATGTA31850.07.6365182
GAAGCAG30500.07.53718479
TGCGTTG33950.07.44278512
CTGCGTT34100.07.41004611
GGAAGCA31050.07.3730818
TGATACC34450.07.36233617
TTGATAC34700.07.33666916
AGTGGTA34150.07.315746314
TACTCTG34700.07.2545427
CAGTGGT34550.07.203554613
CGTTGAT35250.07.195247714
CCCATGT32450.07.17560861
TCTGCGT35450.07.12785710
Produced by FastQC (version 0.11.5)