##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294960_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 304852 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.447351501712305 33.0 31.0 34.0 28.0 34.0 2 31.59438678440686 33.0 31.0 34.0 28.0 34.0 3 31.65132588928398 33.0 31.0 34.0 28.0 34.0 4 35.05510214792752 37.0 35.0 37.0 32.0 37.0 5 35.30930090666946 37.0 35.0 37.0 32.0 37.0 6 35.021879469381865 37.0 35.0 37.0 32.0 37.0 7 34.97872738246756 37.0 35.0 37.0 32.0 37.0 8 35.01385590384842 37.0 35.0 37.0 32.0 37.0 9 36.527659323212575 39.0 37.0 39.0 32.0 39.0 10 36.587780299948825 39.0 37.0 39.0 32.0 39.0 11 36.573406111818194 39.0 37.0 39.0 32.0 39.0 12 36.601993754346374 39.0 37.0 39.0 32.0 39.0 13 36.395952790206394 39.0 37.0 39.0 32.0 39.0 14 37.59861178539094 40.0 38.0 41.0 32.0 41.0 15 37.55457074252424 40.0 38.0 41.0 32.0 41.0 16 37.4676105126422 40.0 38.0 41.0 32.0 41.0 17 37.64348929972577 40.0 38.0 41.0 32.0 41.0 18 37.673936861165416 40.0 38.0 41.0 32.0 41.0 19 37.71701022135331 40.0 38.0 41.0 32.0 41.0 20 37.75501882880874 40.0 38.0 41.0 32.0 41.0 21 37.71387427341792 40.0 38.0 41.0 31.0 41.0 22 37.77607494784355 40.0 38.0 41.0 32.0 41.0 23 37.707133297468935 40.0 38.0 41.0 31.0 41.0 24 37.71708238751919 40.0 38.0 41.0 31.0 41.0 25 36.589233464107174 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31254439545203994 1101 2 -0.38357622104032885 1101 3 -0.07351246579452209 1101 4 -0.4733922489935267 1101 5 -0.10676600729742347 1101 6 -0.11977788566890979 1101 7 -0.18359732760136893 1101 8 -0.07738929289119767 1101 9 0.11205015473959179 1101 10 -0.05194965801057094 1101 11 0.1661765133637516 1101 12 -0.20877367192385776 1101 13 -0.023816485088133277 1101 14 -0.17221488099855264 1101 15 -0.3420126722356045 1101 16 0.10372676388953295 1101 17 -0.2088058085723148 1101 18 -0.08896409724736287 1101 19 -0.4828845644049409 1101 20 -0.664813393579621 1101 21 -0.32076425107528905 1101 22 -0.3273690342691893 1101 23 -0.21166139611664647 1101 24 -0.15866903283607314 1101 25 0.024523264238638376 1102 1 -0.060154222695732784 1102 2 0.013134619606439912 1102 3 -0.04548923044863429 1102 4 -0.10166694912177121 1102 5 -0.11975667795399403 1102 6 -0.1652226011365059 1102 7 0.05421794636951205 1102 8 -0.04532753314065019 1102 9 -0.11624466257124055 1102 10 -0.08469037267348511 1102 11 -0.291273130813579 1102 12 -0.3451379941005328 1102 13 -0.14087837057107322 1102 14 -0.31318100869236787 1102 15 -0.3225140930116268 1102 16 -0.10460677899778403 1102 17 -0.08387307540159128 1102 18 -0.1198942654773063 1102 19 0.13613120243691412 1102 20 0.11912806541904786 1102 21 -0.2596985433276586 1102 22 -0.009492402891410734 1102 23 -0.03797467094251061 1102 24 -0.04314611182116579 1102 25 -0.015675895868987766 1103 1 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1107 8 -0.17776338688231164 1107 9 -0.19297673326601483 1107 10 -0.20104949434445274 1107 11 -0.28044495027899075 1107 12 -0.10524315988270416 1107 13 0.04915522405378425 1107 14 0.01466844843796622 1107 15 -0.007829833797451613 1107 16 -0.045489976816568856 1107 17 0.07772680774753837 1107 18 -0.007551892430903706 1107 19 0.08997024036475665 1107 20 -0.1420871836193669 1107 21 -0.09432048207833077 1107 22 -0.12650394191473424 1107 23 -0.3210605076434163 1107 24 -0.13683129610472378 1107 25 -0.061705400713435665 1108 1 -0.022644041568636197 1108 2 0.07579703282445038 1108 3 -0.028336455364275537 1108 4 0.19003203858330409 1108 5 -0.40578817157382474 1108 6 -0.06309135186372572 1108 7 -0.12671522665146995 1108 8 -0.03939339048851309 1108 9 -0.08415956774131672 1108 10 0.09391083742619344 1108 11 -0.054940081905385796 1108 12 0.16823321948981373 1108 13 0.18918407367513623 1108 14 -0.12981406147908814 1108 15 -0.03549842377220358 1108 16 0.07968131781018428 1108 17 0.0069280338564468025 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4 0.1633301195470267 1110 5 0.02680042626901269 1110 6 0.0661634457269713 1110 7 0.10889200125159704 1110 8 0.09739047279766311 1110 9 0.19241978510922308 1110 10 0.20091111757747626 1110 11 0.19696844870114205 1110 12 0.21617410756937971 1110 13 -0.017672628944282565 1110 14 0.17712668652482932 1110 15 0.12973830406082243 1110 16 0.09824134022228748 1110 17 0.3712822851521409 1110 18 0.38233732859951886 1110 19 0.4793759024009816 1110 20 0.3609108821446583 1110 21 0.16524519402506144 1110 22 0.31364903856706405 1110 23 0.20085790276628757 1110 24 0.10602082276287206 1110 25 -0.017666364314628424 1111 1 0.046311202075433044 1111 2 -0.076248782853515 1111 3 0.17561613997470715 1111 4 -0.01454952921330488 1111 5 0.28088423308719257 1111 6 -0.018434307372203307 1111 7 0.1873075487722602 1111 8 -0.08689736294613937 1111 9 0.11482745556376983 1111 10 0.09513567852788896 1111 11 0.11839148589122317 1111 12 -0.05895825296781254 1111 13 2.606096920843015E-4 1111 14 -0.12617264198756573 1111 15 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0.46337806829204453 2215 9 0.45195750636717236 2215 10 0.3642201834900831 2215 11 0.22320304071163832 2215 12 0.24236573687150553 2215 13 0.1649618443329146 2215 14 0.42057682634892046 2215 15 0.2998120436911904 2215 16 0.4287729842098571 2215 17 0.13481163258766315 2215 18 0.2265389252499901 2215 19 0.26467562587736637 2215 20 0.29642385688258344 2215 21 0.40232513590823515 2215 22 0.2992330970588384 2215 23 0.20876694680696772 2215 24 0.3288566739712948 2215 25 0.3402090906995099 2216 1 0.23769946749900583 2216 2 0.3743619733619532 2216 3 0.2430762861533431 2216 4 0.4473087810997711 2216 5 0.351344206613895 2216 6 0.5986201067622332 2216 7 0.44793283652548155 2216 8 0.23169940846270265 2216 9 0.4234176086484851 2216 10 0.07073060831610434 2216 11 0.16246089196857127 2216 12 0.4167661657706745 2216 13 0.2884130901248625 2216 14 0.5107187599461369 2216 15 0.26532952516183883 2216 16 0.16344384239113907 2216 17 0.4152566851034436 2216 18 0.41701611039029984 2216 19 0.37522160198852106 2216 20 0.4995765442909814 2216 21 0.62272430456796 2216 22 0.6253357195405371 2216 23 0.3914390025638639 2216 24 0.36756900168925455 2216 25 0.2678114677478334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 56.0 10 135.0 11 191.0 12 219.0 13 338.0 14 558.0 15 901.0 16 1271.0 17 1505.0 18 1709.0 19 1842.0 20 1846.0 21 1785.0 22 1672.0 23 1605.0 24 1603.0 25 1610.0 26 1673.0 27 1710.0 28 1856.0 29 2261.0 30 2897.0 31 3806.0 32 5064.0 33 7037.0 34 10554.0 35 15780.0 36 25973.0 37 51887.0 38 155500.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.188629219087375 19.36301050711084 12.887781956120836 21.560578317680953 2 13.873086487798798 24.011338359481112 35.95106395543395 26.164511197286146 3 20.866669947022256 31.1600977545966 28.7756072758287 19.197625022552444 4 12.957805616514298 21.667962381622495 31.436013003073633 33.93821899878957 5 10.368309868395157 40.0755120517497 34.40095521761379 15.155222862241349 6 29.65241211882617 33.98979709003822 17.702212817610683 18.655577973524924 7 20.67659060790154 37.278745095981 22.78417067954286 19.2604936165746 8 27.452993583771796 32.475758728825795 21.296891606418853 18.77435608098356 9 27.284715206067204 16.28724758243344 21.36020101557477 35.06783619592458 10 18.525710836733893 31.47199296707911 29.474958340440605 20.527337855746396 11 26.12185585136394 27.78987836720769 19.267382205135608 26.820883576292758 12 24.54535315497356 32.76868775668193 27.975870258354874 14.710088829989635 13 30.39310878721478 22.67231312243318 24.551913715507855 22.382664374844186 14 22.48107278285857 27.687205594846027 27.913872961305813 21.917848660989595 15 32.10836733890544 22.65000721661659 26.2107514466036 19.03087399787438 16 17.077795126815634 28.53712621206356 34.1405009644024 20.24457769671841 17 22.00674425622925 29.0973980816921 29.720651332449844 19.175206329628804 18 22.195032343563433 22.281959770642803 35.166244603938964 20.3567632818548 19 20.11566268221957 28.326532218912785 37.171807959272044 14.385997139595608 20 21.967124923323198 23.17722428333842 35.24869526424384 19.60695552909454 21 18.90064687126868 26.37279729180061 36.86378964218703 17.862766194743678 22 20.737343817143458 25.29301199602429 31.004326703865164 22.965317482967087 23 17.24508942044008 25.257173972944248 35.64319735478199 21.85453925183368 24 19.949352472675265 30.00603571569155 32.669295264587404 17.37531654704578 25 20.095652972589978 22.924894703003424 31.361775550102998 25.617676774303593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1507.0 1 1507.0 2 1302.5 3 1098.0 4 1098.0 5 1098.0 6 2557.0 7 4016.0 8 4016.0 9 4016.0 10 3831.5 11 3647.0 12 3647.0 13 3647.0 14 2948.5 15 2250.0 16 2250.0 17 2250.0 18 3610.5 19 4971.0 20 4971.0 21 4971.0 22 7650.0 23 10329.0 24 10329.0 25 10329.0 26 13962.0 27 17595.0 28 17595.0 29 17595.0 30 19676.5 31 21758.0 32 21758.0 33 21758.0 34 21659.0 35 21560.0 36 21560.0 37 21560.0 38 20012.0 39 18464.0 40 18464.0 41 18464.0 42 20392.0 43 22320.0 44 22320.0 45 22320.0 46 47324.5 47 72329.0 48 72329.0 49 72329.0 50 63161.0 51 53993.0 52 53993.0 53 53993.0 54 38823.5 55 23654.0 56 23654.0 57 23654.0 58 17652.5 59 11651.0 60 11651.0 61 11651.0 62 9564.5 63 7478.0 64 7478.0 65 7478.0 66 5646.5 67 3815.0 68 3815.0 69 3815.0 70 2681.0 71 1547.0 72 1547.0 73 1547.0 74 1094.5 75 642.0 76 642.0 77 642.0 78 408.0 79 174.0 80 174.0 81 174.0 82 111.5 83 49.0 84 49.0 85 49.0 86 26.5 87 4.0 88 4.0 89 4.0 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03477097083174786 2 0.015089289228871716 3 0.0022961961870022174 4 3.280280267146025E-4 5 0.0 6 0.012137036988440292 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 3.280280267146025E-4 21 0.0 22 3.280280267146025E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 304852.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.22243973132602 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83595387442902 25.952913609352674 2 14.082846432367594 9.638987265544392 3 4.737916618589547 4.864291977951862 4 2.0321851152002717 2.781853305113565 5 1.031258288764546 1.7646087317337542 6 0.591404566229796 1.2143584274778123 7 0.3480592008434074 0.8338004516657905 8 0.22140139704971873 0.6061516773572305 9 0.16156316608595303 0.497617714276087 >10 0.7719128932781613 4.950579450157205 >50 0.07579506443177503 1.9000259079084485 >100 0.06582202963812042 4.627366850983069 >500 0.007978427834923687 2.1836475226869503 >1k 0.02991910438096383 20.999945048984898 >5k 0.003989213917461844 9.32060490732384 >10k+ 0.001994606958730922 7.86324715148244 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 11862 3.8910684528886152 No Hit GTACATGGGAAGCAGTGGTATCAAC 11177 3.666369254589112 No Hit GTATCAACGCAGAGTACTTTTTTTT 9153 3.0024405285187563 No Hit GGTATCAACGCAGAGTACTTTTTTT 6932 2.2738902811856243 No Hit TATCAACGCAGAGTACTTTTTTTTT 5847 1.917979872200281 No Hit GAGTACATGGGAAGCAGTGGTATCA 5377 1.7638066996444175 No Hit CATGTACTCTGCGTTGATACCACTG 4674 1.5332029968640521 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3744 1.228136932019472 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3622 1.1881175127602903 No Hit GCGTTGATACCACTGCTTCCCATGT 3597 1.1799168120924253 No Hit TATCAACGCAGAGTACATGGGAAGC 3227 1.0585464422080222 No Hit GGTATCAACGCAGAGTACATGGGAA 3021 0.9909726687048142 No Hit ACTCTGCGTTGATACCACTGCTTCC 2975 0.9758833794759425 No Hit GTATCAACGCAGAGTACATGGGAAG 2935 0.9627622584073583 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2892 0.9486570532586305 No Hit GTGGTATCAACGCAGAGTACATGGG 2387 0.7830028997677562 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2194 0.7196934906118378 No Hit GCTTCCCATGTACTCTGCGTTGATA 1961 0.6432629603873354 No Hit ATACCACTGCTTCCCATGTACTCTG 1628 0.5340296274913728 No Hit GATACCACTGCTTCCCATGTACTCT 1610 0.5281251230105101 No Hit CATGGGAAGCAGTGGTATCAACGCA 1569 0.5146759739152114 No Hit CAGTGGTATCAACGCAGAGTACATG 1557 0.5107396375946361 No Hit GTACTCTGCGTTGATACCACTGCTT 1555 0.5100835815412069 No Hit ACATGGGAAGCAGTGGTATCAACGC 1461 0.47924894703003423 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1460 0.4789209190033197 No Hit GGGAAGCAGTGGTATCAACGCAGAG 1419 0.46547176990802097 No Hit CTGCTTCCCATGTACTCTGCGTTGA 1364 0.4474302284387178 No Hit GCAGAGTACATGGGAAGCAGTGGTA 1311 0.43004474302284385 No Hit GCAGTGGTATCAACGCAGAGTACAT 1267 0.41561150984740136 No Hit GTGGTATCAACGCAGAGTACTTTTT 1259 0.4129872856336846 No Hit TACCACTGCTTCCCATGTACTCTGC 1213 0.3978979964048128 No Hit CCACTGCTTCCCATGTACTCTGCGT 1157 0.3795284269087951 No Hit GTTGATACCACTGCTTCCCATGTAC 1139 0.37362392242793224 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1125 0.3690315300539278 No Hit ATCAACGCAGAGTACTTTTTTTTTT 1105 0.36247096951963576 No Hit CTTCCCATGTACTCTGCGTTGATAC 1101 0.3611588574127773 No Hit ATGGGAAGCAGTGGTATCAACGCAG 975 0.3198273260467374 No Hit AAGCAGTGGTATCAACGCAGAGTAC 963 0.3158909897261622 No Hit CCATGTACTCTGCGTTGATACCACT 924 0.3030978966842927 No Hit TTCCCATGTACTCTGCGTTGATACC 843 0.2765276265204099 No Hit TGATACCACTGCTTCCCATGTACTC 808 0.2650466455853988 No Hit ATCAACGCAGAGTACATGGGAAGCA 752 0.24667707608938108 No Hit GTACATGGTAAGCAGTGGTATCAAC 591 0.19386456378833009 No Hit GGAAGCAGTGGTATCAACGCAGAGT 542 0.17779119047931455 No Hit CAACGCAGAGTACTTTTTTTTTTTT 480 0.15745345282300918 No Hit AACGCAGAGTACTTTTTTTTTTTTT 476 0.1561413407161508 No Hit ACCATGTACTCTGCGTTGATACCAC 444 0.14564444386128353 No Hit CTGCGTTGATACCACTGCTTCCCAT 383 0.12563473423169275 No Hit TGGGAAGCAGTGGTATCAACGCAGA 376 0.12333853804469054 No Hit TCAACGCAGAGTACTTTTTTTTTTT 373 0.12235445396454672 No Hit CTCTGCGTTGATACCACTGCTTCCC 367 0.12038628580425911 No Hit AAAAAGTACTCTGCGTTGATACCAC 364 0.11940220172411531 No Hit TCAACGCAGAGTACATGGGAAGCAG 358 0.1174340335638277 No Hit AGTGGTATCAACGCAGAGTACATGG 323 0.1059530526288166 No Hit CCCCATGTACTCTGCGTTGATACCA 322 0.105625024602102 No Hit GTACATGGAAAGCAGTGGTATCAAC 319 0.1046409405219582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCCTA 25 0.0060236086 18.998688 16 GTGAGCT 55 0.0030565525 12.092057 4 GAACAAA 110 4.890808E-7 11.232024 1 CCCAGCT 110 5.9976246E-6 10.362921 9 CCAGCTA 95 0.0018158652 8.999378 10 TTTTGTG 110 7.1058975E-4 8.635767 19 TCCCAGC 140 1.0310539E-4 8.142295 8 CCATGTA 3185 0.0 7.636518 2 GAAGCAG 3050 0.0 7.5371847 9 TGCGTTG 3395 0.0 7.442785 12 CTGCGTT 3410 0.0 7.410046 11 GGAAGCA 3105 0.0 7.373081 8 TGATACC 3445 0.0 7.362336 17 TTGATAC 3470 0.0 7.336669 16 AGTGGTA 3415 0.0 7.3157463 14 TACTCTG 3470 0.0 7.254542 7 CAGTGGT 3455 0.0 7.2035546 13 CGTTGAT 3525 0.0 7.1952477 14 CCCATGT 3245 0.0 7.1756086 1 TCTGCGT 3545 0.0 7.127857 10 >>END_MODULE