FastQCFastQC Report
Wed 25 May 2016
SRR1294959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4834029
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC110130.22782238170271632No Hit
CCCATGTACTCTGCGTTGATACCAC102530.212100506637424No Hit
GTATCAACGCAGAGTACTTTTTTTT63070.13047087636420882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG752.0753722E-411.4001015
TAACCCG1106.842062E-59.5000825
TACCGTG1151.1138571E-49.0852487
TCGGAAC850.0074509748.93949513
TCTATAC7650.08.8178923
TATAACG1201.7632672E-48.7092212
GTGTAGG5900.08.7074341
TCTGTCG3900.08.5240398
TATACTG8550.08.4445185
GTATTAA11050.08.3513731
ATTATAC9450.08.3447663
TAGGACT6200.08.27520754
TACACCG1150.00111121228.26094155
TAATACT9900.08.2535374
GTCTAGA4850.08.2386481
CTTAGAC4500.08.2341723
GTCTAAT5200.08.232991
GTATTAC5550.08.2280451
GTAGGAC3700.08.2170533
TTATACT9900.08.1575664
Produced by FastQC (version 0.11.5)