FastQCFastQC Report
Wed 25 May 2016
SRR1294959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4834029
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT181410.37527702047298434No Hit
CCCATGTACTCTGCGTTGATACCAC111620.2309046966826223No Hit
GTACATGGGAAGCAGTGGTATCAAC107600.22258865224019136No Hit
TATCAACGCAGAGTACTTTTTTTTT103420.21394162095428057No Hit
GGTATCAACGCAGAGTACTTTTTTT101650.21028007899828488No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53400.11046685901139608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT905.408365E-712.6675354
ATCGACC701.0924909E-412.2134828
TACACCG1158.821462E-710.7398675
TATACCG856.5941503E-410.0595135
TAAATCG1106.837584E-59.5006515
GCGTGCG900.00111508079.4993749
CTTACCG900.00111508079.4993749
CGTTCCG1252.7589156E-59.11939910
TAACGCT850.0074353128.941794
CTAATAC8000.08.9068613
TACCGTG1502.707331E-68.8660827
GACTGCG1107.15389E-48.6357957
TGCGTTG30500.08.5650112
GAGTACG1000.00289565748.5535951
TTAGACT5000.08.5505874
CCGTACA1000.0029084318.54943717
CTGCGTT31150.08.41678111
CTCTGCG31700.08.3606479
TCTGCGT31950.08.35469210
GTATTAT10150.08.3335511
Produced by FastQC (version 0.11.5)