FastQCFastQC Report
Wed 25 May 2016
SRR1294958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3840974
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC116590.3035427993003858No Hit
CCCATGTACTCTGCGTTGATACCAC102850.2677706227639135No Hit
GAGTACATGGGAAGCAGTGGTATCA51380.13376815359854036No Hit
GTATCAACGCAGAGTACTTTTTTTT45820.11929265858087038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC500.001502458913.29746
AGCGTAA550.003073755412.088860510
ACACCGT1604.5474735E-1111.2790466
TACACCG1355.69471E-810.5556945
TTAGACG750.002646510510.1345224
GTCTAGG3150.09.9671891
TATACCG800.0045175159.5001245
GCACCGT1252.7626655E-59.1182176
ACGACAG950.00182451279.0001175
GTATTAG6400.08.77084351
AGCACCG1304.3713815E-58.7693455
AGACCGT1000.00291156238.5483286
GACCGTG1356.81842E-58.4427947
GTCTTAG3950.08.4302451
GTATTAA7700.08.4020931
GTGCGTC1150.0011131878.25936311
GTCTAAG3350.08.2361161
CTTATAC5700.08.1675193
ACGACTG1050.00451444658.1429635
GTCTAGA4100.08.1218221
Produced by FastQC (version 0.11.5)