FastQCFastQC Report
Wed 25 May 2016
SRR1294958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3840974
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT126450.3292133714000668No Hit
GTACATGGGAAGCAGTGGTATCAAC118260.3078906548182831No Hit
CCCATGTACTCTGCGTTGATACCAC110540.2877915861966262No Hit
GGTATCAACGCAGAGTACTTTTTTT73110.19034234545716788No Hit
TATCAACGCAGAGTACTTTTTTTTT69580.18115196822472635No Hit
GAGTACATGGGAAGCAGTGGTATCA50700.13199776931580373No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39110.10182313132033698No Hit
GCGTTGATACCACTGCTTCCCATGT38410.10000067691163751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG856.593952E-410.0594385
TAGACAG4900.09.8883585
ATACCGT900.00111367799.500586
TATACCG1106.8371664E-59.5005795
ACCGTAT900.00111513749.4992198
TGAGCGG1151.1124164E-49.08620910
CTGAGCG950.00182609648.999269
GGGCGCG950.00182609648.9992611
GTGCTCG850.0074439328.9404429
GTCCACG1401.0773769E-58.82702351
TAGCACC3100.08.5811694
CCGGGTT2356.184564E-108.48866417
GTATTAG5500.08.4690041
CTAATAC4600.08.4679073
GTCCAGA5400.08.44980051
TCTATAC4650.08.3768553
CTGTGCG1252.747467E-48.3593129
CTACACT5350.08.3463034
CTTATAC5750.08.26137353
CACACCG1502.5860287E-58.2338355
Produced by FastQC (version 0.11.5)