FastQCFastQC Report
Wed 25 May 2016
SRR1294957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3749386
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC224990.6000715850541929No Hit
CCCATGTACTCTGCGTTGATACCAC205660.5485164770978501No Hit
GAGTACATGGGAAGCAGTGGTATCA91460.2439332733412884No Hit
GCGTTGATACCACTGCTTCCCATGT71060.1895243647893282No Hit
CATGTACTCTGCGTTGATACCACTG67620.18034952922958586No Hit
GTATCAACGCAGAGTACATGGGAAG65990.1760021507521498No Hit
GTATCAACGCAGAGTACTTTTTTTT59670.15914605751448369No Hit
TATCAACGCAGAGTACATGGGAAGC49150.13108813016317872No Hit
ACGCAGAGTACATGGGAAGCAGTGG47400.12642069928249586No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA43970.11727253475635743No Hit
GGTATCAACGCAGAGTACATGGGAA42300.1128184721445058No Hit
GCTTCCCATGTACTCTGCGTTGATA41480.11063144738898582No Hit
ACTCTGCGTTGATACCACTGCTTCC40650.10841775159986194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCG1203.6379788E-1214.250632
GTATAGA5700.09.5132461
TCTAGAC3100.09.1939543
GTATTAG6050.09.1201361
GCCGCGG2002.6575435E-99.0253993
GTCTACG850.00735430938.9536441
GTCCTAT2701.8189894E-128.808561
CCCGTGC1201.7681814E-48.7068598
TGGACAG5200.08.5862315
CTTATAC4300.08.39572053
TTATACT6550.08.2676524
CTGCTCG1502.5938785E-58.231839
GGACTGT4650.08.1706616
GTCTAGA3850.08.154211
TTCTCGA1050.00451312778.1432182
AACACGG1659.756479E-68.0603175
CCTACAC4250.08.0474153
CGTGCCA1909.195501E-77.99864610
TATACTG6950.07.9277735
CTAGACC3001.8189894E-117.9171234
Produced by FastQC (version 0.11.5)