FastQCFastQC Report
Wed 25 May 2016
SRR1294957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3749386
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC219650.5858292531097092No Hit
CCCATGTACTCTGCGTTGATACCAC218430.5825753870100331No Hit
GTATCAACGCAGAGTACTTTTTTTT163670.43652480699506535No Hit
GAGTACATGGGAAGCAGTGGTATCA94490.25201459652327074No Hit
TATCAACGCAGAGTACTTTTTTTTT94300.2515078468847966No Hit
GGTATCAACGCAGAGTACTTTTTTT91920.2451601408870679No Hit
GCGTTGATACCACTGCTTCCCATGT72840.19427180877082273No Hit
CATGTACTCTGCGTTGATACCACTG70110.18699061659695748No Hit
GTATCAACGCAGAGTACATGGGAAG66390.17706899209630592No Hit
ACGCAGAGTACATGGGAAGCAGTGG53180.14183655670555126No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51180.13650234998477084No Hit
TATCAACGCAGAGTACATGGGAAGC51150.13642233688395913No Hit
ACTCTGCGTTGATACCACTGCTTCC44340.11825936299970181No Hit
GCTTCCCATGTACTCTGCGTTGATA44150.11775261336122768No Hit
GGTATCAACGCAGAGTACATGGGAA41870.111671617699538No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39180.10449710966008835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTC550.003070901612.0903499
CCGTGCA803.7749685E-410.6870059
GTATAAG4700.09.9080251
CCGGGAC800.0045167069.500323
TGGTCGA900.00111475819.4995610
TCTGTCG2850.09.3329018
GTCTTAT4600.09.0904151
TTATACT6950.09.0217674
GCCTAAC1701.6771264E-78.9445791
ACACCGC850.00743760638.9413596
ATTAGAC3250.08.76952653
GTATTAG5600.08.6550791
CTGTCGC3500.08.142489
CTATTAG4100.08.1128431
TAGACAG5800.08.0260265
GTATTAA8300.08.0150981
GTCCTAG3105.456968E-127.97077371
TTCGTCT1553.902996E-57.967372418
TAGACTG4550.07.93422755
CCAGGAC5150.07.9323063
Produced by FastQC (version 0.11.5)