FastQCFastQC Report
Wed 25 May 2016
SRR1294956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6027828
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC120590.20005547603548077No Hit
CCCATGTACTCTGCGTTGATACCAC108400.17983260305370358No Hit
GTATCAACGCAGAGTACTTTTTTTT63860.1059419744558073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG1151.0277698E-59.9129955
ATACCGT1252.7634967E-59.1182156
GTCGTAA950.00180427019.011331
GTATTAG9000.08.9835181
ACCGTCC1651.0544918E-68.6346738
TATACCG1000.00290703568.5499595
GTATAGG6550.08.4228041
GTCTTAG5550.08.3979461
TAGGACG1252.7404362E-48.3614884
CTTACAC8600.08.2862213
CTGTCGC4850.08.2250579
CTAAGAC5800.08.1909763
TAAGACT7350.08.1443064
CTACACA11100.08.0464764
GTCCAAG6650.08.0100721
TAGGACC4050.07.97672754
TTAGACC4550.07.93547734
ACGGGTC1200.00169215137.9166295
TCTGTCG4950.07.86714658
GTCCTAT5200.07.86565881
Produced by FastQC (version 0.11.5)