FastQCFastQC Report
Wed 25 May 2016
SRR1294956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6027828
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT174110.2888436763623647No Hit
GTACATGGGAAGCAGTGGTATCAAC114890.1905993336239853No Hit
CCCATGTACTCTGCGTTGATACCAC114660.1902177699828197No Hit
GGTATCAACGCAGAGTACTTTTTTT98980.16420508348944263No Hit
TATCAACGCAGAGTACTTTTTTTTT95330.15814983440137972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC855.324426E-511.1773153
TCGTCCG600.005882941711.0823329
CGTGTCG600.005882941711.0823329
GTCGTAC803.751535E-410.6944291
ATAACGC800.00451581979.5007173
AGACCGT1804.1873136E-99.5004826
AATACCG1054.4939466E-49.0480775
TGGACGG1606.7462133E-78.9067015
TAGACAG10200.08.7553455
TAGACCG1201.7644397E-48.7087755
TCGTCTA1107.1562757E-48.63558314
TCTAGAC4200.08.5958873
CTAGGAC5650.08.4077153
CTAAGAC6000.08.3923013
GTCTACG1252.7265606E-48.365421
CTACACT8550.08.33375554
GTATTAA11300.08.244281
CCGTCTG1856.1354694E-78.2154739
CGTTATA1050.0045049188.1450262
TAACGCT1050.00451367778.143274
Produced by FastQC (version 0.11.5)