FastQCFastQC Report
Wed 25 May 2016
SRR1294955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3787440
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC170150.4492480408930571No Hit
CCCATGTACTCTGCGTTGATACCAC160640.4241387322307416No Hit
GAGTACATGGGAAGCAGTGGTATCA70250.18548148617535856No Hit
GCGTTGATACCACTGCTTCCCATGT55040.14532243415077203No Hit
CATGTACTCTGCGTTGATACCACTG54850.1448207760386963No Hit
GTATCAACGCAGAGTACTTTTTTTT53150.1403322560885453No Hit
GTATCAACGCAGAGTACATGGGAAG50350.13293939970006127No Hit
TATCAACGCAGAGTACATGGGAAGC41170.10870139196924572No Hit
ACGCAGAGTACATGGGAAGCAGTGG41010.10827894303276091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA39360.1039224383752614No Hit
GGTATCAACGCAGAGTACATGGGAA37900.10006759182983756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG600.005879366311.0830635
AGTACCG750.00264960810.1330865
CCGATCT750.002653386210.1313479
GCTGTCG856.60951E-410.056858
ACACCGT951.6484973E-49.9980396
CCTACAC3600.09.5014023
TACACCG800.0045188859.4997685
AACCGTG800.00452529279.4981377
GTACCGT900.00111629129.4981376
TACCCCG800.00452529279.4981377
CCGCGTG800.00452529279.4981379
GTCTAAG3500.08.9698451
ACCGTCC850.00745084978.9394238
GTATTAG4650.08.7974051
CAATACT4650.08.5819114
TATACTG5500.08.463435
GACGAAC1150.00111318398.25935813
ATAGGCG1502.5887604E-58.2331335
TTACACC3950.08.1784224
CTTACAC4650.08.1732483
Produced by FastQC (version 0.11.5)