FastQCFastQC Report
Wed 25 May 2016
SRR1294955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3787440
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC178080.4701856663075851No Hit
GTACATGGGAAGCAGTGGTATCAAC170600.4504361785269206No Hit
GTATCAACGCAGAGTACTTTTTTTT156750.41386794246245484No Hit
GGTATCAACGCAGAGTACTTTTTTT91830.24245928648374626No Hit
TATCAACGCAGAGTACTTTTTTTTT89160.23540966985615613No Hit
GAGTACATGGGAAGCAGTGGTATCA70250.18548148617535856No Hit
GCGTTGATACCACTGCTTCCCATGT58530.15453710157784678No Hit
CATGTACTCTGCGTTGATACCACTG57850.1527416935977864No Hit
GTATCAACGCAGAGTACATGGGAAG49710.13124960395412202No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46330.12232537017088059No Hit
ACGCAGAGTACATGGGAAGCAGTGG44590.11773123798660837No Hit
TATCAACGCAGAGTACATGGGAAGC41850.11049679994930614No Hit
ACTCTGCGTTGATACCACTGCTTCC38820.10249667321462518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG3650.010.1498888
GCGTGCG750.002650800410.1325389
AGGACCG1106.835688E-59.5007595
TACGACC800.0045150039.5007594
CTGTCGC4200.09.4992539
CTAGTAC2501.8189894E-129.120853
GTATTAA6900.09.0914041
AGACCGT1054.4916538E-49.0483426
TCGCACT850.00744366558.94047511
TAGACTG4000.08.7882025
GTATAAC3600.08.7125961
TGTCGCC4500.08.65487610
GTCCTGG6700.08.6534871
GTCTAGC1651.0436688E-68.640591
GTACTAC2553.8198777E-118.5728221
TTAGACA4800.08.5110974
GTATAAA7150.08.5076581
CTAGACA3650.08.32943254
GTCGCCC4350.08.298211
GTGTTAG5100.08.200091
Produced by FastQC (version 0.11.5)