FastQCFastQC Report
Wed 25 May 2016
SRR1294954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4433214
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58270.13143962822457927No Hit
CCCATGTACTCTGCGTTGATACCAC52920.11937163421391343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT658.043748E-411.6896756
CTACGCT752.071541E-411.4023254
TACGCTG1252.5726204E-69.8801175
AAGACCG900.0011142499.5001135
GTCCTAT3900.09.2700221
GTATAGA6000.09.0382711
ATTAGAC4150.08.929433
TAGACAG6100.08.8771555
GTCCTAG5000.08.7528521
TCTGTCG3050.08.7193488
TTAGGAC4250.08.7193263
TAACGCA1107.1344234E-48.6381244
CGTATAC1107.135225E-48.6380263
GTATTAG7050.08.6367951
ACTGCCG1107.164937E-48.6344198
GTTCTAG4800.08.5229311
GTCTTAG4600.08.4798431
CTAGGAC4600.08.4690223
GTCTTAT3750.08.3722931
TTAGACA5000.08.3617044
Produced by FastQC (version 0.11.5)