FastQCFastQC Report
Wed 25 May 2016
SRR1294954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4433214
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT122010.27521793443763376No Hit
GGTATCAACGCAGAGTACTTTTTTT72850.16432773152841257No Hit
TATCAACGCAGAGTACTTTTTTTTT68020.15343270142158713No Hit
CCCATGTACTCTGCGTTGATACCAC57870.13053734829854818No Hit
GTACATGGGAAGCAGTGGTATCAAC57090.12877790244278756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT1353.7289283E-1011.9633396
GGCGTAG1002.7369434E-49.5070551
TTCAACG1002.7503146E-49.5024422
ACACCGT1752.6726411E-89.2288626
TAGACAG5400.09.1484365
TGGACCG1252.7555907E-59.1202875
TAGTACG1304.3697564E-58.7697054
GTATTAA7550.08.6885671
TTAGACA4450.08.3262924
GTATCAA75500.08.3233951
ACGGAAC1951.5359183E-78.2827793
TAGGACC3001.8189894E-128.2337784
TAGACTG6000.08.2335925
GTCTAGG4850.08.2329131
GTCTAAC1856.056507E-78.2223181
GTTCTAG5150.08.1225321
TTAGACT3650.08.0689294
GTCTTAC3800.08.005941
ACTATAC5600.07.9739153
CTAGACA5900.07.89025644
Produced by FastQC (version 0.11.5)