FastQCFastQC Report
Wed 25 May 2016
SRR1294953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727206
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC500.001482928813.3206141
TAAGACG802.8642424E-511.8770314
TCGCACT600.00587118311.0852314
TACGAGT750.002645524710.1350674
GTCTAGA4400.09.5147241
AGTACCG800.00451747659.5002175
GCGCCCC800.00452537379.49820513
TCGTACC950.00182205319.001542
GTCTTGC8250.08.9957391
GTATTAA9100.08.9919371
GTCTAGG5100.08.9550351
GTACTAG3250.08.7828221
GTATCAA43700.08.6873571
TATTCCG1752.6280213E-78.6859135
GTCCTGG10300.08.5909651
AGGACCG1000.00290606998.5501965
TAGACAG7400.08.4731665
ATTAGAC4150.08.4713293
ATAATAC8300.08.4713293
CCTACAC4650.08.3777773
Produced by FastQC (version 0.11.5)