FastQCFastQC Report
Wed 25 May 2016
SRR1294953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727206
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT116580.246615019527391No Hit
TATCAACGCAGAGTACTTTTTTTTT63260.13382111970580507No Hit
GGTATCAACGCAGAGTACTTTTTTT59780.12645947733185312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG8450.09.6689795
GACCCGC800.0045170229.5003346
ACAGTCG800.0045209659.4993298
GTATCAA58400.09.358281
TAGACCG1054.4941902E-49.0479365
GTACTAG3400.08.9456541
GGTATCA31900.08.9088481
GACAGCG1401.0872067E-58.8208057
TTGGACA6300.08.29394154
GTCCTAT4250.08.274731
GTCTTAC4250.08.274731
GACTGCG1502.5907546E-58.2327517
GTATTAA8350.08.1957191
GTAGGAC2803.45608E-118.1432293
TAATACT9600.08.0159064
GTCTTAG4650.07.9717321
TGCGTTG10750.07.95292612
GTCTCGG1200.00168358037.9206311
CTTACAC6850.07.90547043
GTTCTAT5300.07.8907421
Produced by FastQC (version 0.11.5)