FastQCFastQC Report
Wed 25 May 2016
SRR1294952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396209
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59440.17501867523465134No Hit
GGTATCAACGCAGAGTACTTTTTTT36270.10679554762383586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGC551.131096E-515.54254910
GGACCGT550.003074373412.08846956
GGTATCA30150.011.1391941
TCGGCTT750.002649173110.13323117
CAGTGCG856.6103676E-410.0566469
GACCGTG800.0045252989.4980847
ATCGTTC800.0045252989.4980848
TCTAGAC3350.09.3588043
GTATCAA56450.09.2696881
TACCGTG950.00182796788.9981857
GGCCCGG850.00734878478.9544191
CTAAGAC3050.08.7218673
CCGTGTG1107.163228E-48.6344949
GTCTTGG5250.08.5173581
GTCTAAT3950.08.4301881
GTCCTAG3500.08.4267481
GTCTAGG3500.08.4267481
TAGGCAG3950.08.4175135
TTATACT6900.08.3989614
CCTACAC3400.08.3828863
Produced by FastQC (version 0.11.5)