FastQCFastQC Report
Wed 25 May 2016
SRR1294952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396209
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT175050.5154276429984138No Hit
GGTATCAACGCAGAGTACTTTTTTT109790.3232722132236267No Hit
TATCAACGCAGAGTACTTTTTTTTT95760.28196144583563615No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52700.1551730179149752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.012.3450791
TATACCG907.457824E-611.6124965
GCGAGAC856.5891055E-410.0601723
TACACCG856.589998E-410.0600245
GTATCAA73000.09.6744631
TCCGTAC800.0045127829.5012743
AAACCGA900.00111301369.5011346
ATACCGT900.00111301369.5011346
ACCGTTC800.00452156049.4990368
GTCCTAG3100.09.1985741
ACACCGT950.00182263699.0010746
CTAATAC4450.08.9674953
CCGTACC850.00743126738.9422444
GTCATAG3200.08.91111851
GTCTTAG3850.08.8879731
TGTACTG3750.08.8677255
TTAGACT3000.08.8677244
TTACACT7000.08.8224814
CTAGACA4800.08.70937254
TACCGTG1201.7667541E-48.7074497
Produced by FastQC (version 0.11.5)