FastQCFastQC Report
Wed 25 May 2016
SRR1294951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558562
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC154770.4349228705302873No Hit
CCCATGTACTCTGCGTTGATACCAC135640.38116520099972967No Hit
GAGTACATGGGAAGCAGTGGTATCA63850.1794264087572452No Hit
GTATCAACGCAGAGTACTTTTTTTT62950.17689729727906947No Hit
GTATCAACGCAGAGTACATGGGAAG51410.14446846788112727No Hit
GCGTTGATACCACTGCTTCCCATGT48700.13685303220795367No Hit
CATGTACTCTGCGTTGATACCACTG45510.127888737079753No Hit
ACGCAGAGTACATGGGAAGCAGTGG37120.10431179785542588No Hit
GGTATCAACGCAGAGTACTTTTTTT36010.1011925603656758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG550.00306911712.0912595
TTGTACG600.005870149511.085374
TCGGGAC600.00587076411.0852143
GGACCGA700.001494664510.8550146
GTCTAGA3450.010.7557831
AGTATCG750.002648370610.1336265
TAAAGCG900.00111396179.5002755
TGGACCG900.00111396179.5002755
AGGCCCG1502.650122E-79.5002755
GTCCTAG3950.08.6716541
AGAGCCG1107.145975E-48.6366145
AGACCGT1107.161993E-48.634676
ACACCGT1554.22168E-68.5789626
GTCTTAG3550.08.5766591
CTAGCAC4050.08.2112693
CCTACAC4200.08.14423753
TCTGTCG3150.08.1412598
TTATACG1304.1796282E-48.0399394
GACTCCG1304.1967264E-48.0368847
AGTGGTA45300.08.01025514
Produced by FastQC (version 0.11.5)