FastQCFastQC Report
Wed 25 May 2016
SRR1294951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558562
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT182980.5141964647517734No Hit
GTACATGGGAAGCAGTGGTATCAAC152440.42837528192567675No Hit
CCCATGTACTCTGCGTTGATACCAC149980.42146237721866303No Hit
GGTATCAACGCAGAGTACTTTTTTT107670.3025660365057571No Hit
TATCAACGCAGAGTACTTTTTTTTT103210.290032884069464No Hit
GAGTACATGGGAAGCAGTGGTATCA65760.18479374533870704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52690.14806542642786608No Hit
GCGTTGATACCACTGCTTCCCATGT51750.14542390999510477No Hit
GTATCAACGCAGAGTACATGGGAAG49220.1383142966175663No Hit
CATGTACTCTGCGTTGATACCACTG46770.13142949314919902No Hit
ACGCAGAGTACATGGGAAGCAGTGG38310.10765584525434713No Hit
TATCAACGCAGAGTACATGGGAAGC36700.1031315458322772No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35760.10049002939951587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA655.454678E-513.1542756
ACCGTCC752.0765173E-411.399258
ATAGGAC2750.010.364123
GATACCG750.002648291410.1336635
CTAGTAC2350.010.1068543
GTCCTAG3400.09.504851
GTACCGT800.0045166539.5003096
TGTACCG800.0045166539.5003095
GTCCAAG3450.09.0915961
GCGCCAT950.00182575448.99940811
ATCGACC850.00744270678.9405888
AGTACTG4350.08.7359165
TAGGACC2751.8189894E-128.6366444
TACACCG1107.1457226E-48.6366445
TGCACCG1107.1457226E-48.6366445
GTATTAA6400.08.61377051
TAGGACA3650.08.589324
GTGTTAC3800.08.504341
TAAGACA6300.08.29392054
ATACCGT1150.0011107718.2611396
Produced by FastQC (version 0.11.5)