FastQCFastQC Report
Wed 25 May 2016
SRR1294950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833735
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC281423.3754130509094615No Hit
CCCATGTACTCTGCGTTGATACCAC268903.2252454317019197No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA158261.8982050651585936No Hit
GAGTACATGGGAAGCAGTGGTATCA131351.5754406376126708No Hit
CATGTACTCTGCGTTGATACCACTG102911.234325055323334No Hit
GCGTTGATACCACTGCTTCCCATGT93311.1191805549725031No Hit
ACGCAGAGTACATGGGAAGCAGTGG83831.0054753608760578No Hit
GTATCAACGCAGAGTACATGGGAAG81490.9774088889155427No Hit
GGTATCAACGCAGAGTACATGGGAA79420.9525808560273947No Hit
TATCAACGCAGAGTACATGGGAAGC79380.952101087275933No Hit
ACTCTGCGTTGATACCACTGCTTCC69800.8371964713008331No Hit
GTGGTATCAACGCAGAGTACATGGG54630.6552441723089472No Hit
GCTTCCCATGTACTCTGCGTTGATA51350.6159031346890799No Hit
GTACTCTGCGTTGATACCACTGCTT45740.548615567296563No Hit
GTATCAACGCAGAGTACTTTTTTTT41550.49835979058094No Hit
ATACCACTGCTTCCCATGTACTCTG40140.4814479420919117No Hit
GATACCACTGCTTCCCATGTACTCT39490.47365169988065753No Hit
GGGAAGCAGTGGTATCAACGCAGAG35990.43167193412775046No Hit
CAGTGGTATCAACGCAGAGTACATG35760.4289132638068451No Hit
CTGCTTCCCATGTACTCTGCGTTGA33750.4048048840458899No Hit
GCAGTGGTATCAACGCAGAGTACAT33640.40348551997936993No Hit
GCAGAGTACATGGGAAGCAGTGGTA33540.4022860981007155No Hit
CATGGGAAGCAGTGGTATCAACGCA33270.3990476590283483No Hit
TACCACTGCTTCCCATGTACTCTGC31950.3832152902301091No Hit
GGTATCAACGCAGAGTACTTTTTTT31600.3790173136548184No Hit
ACATGGGAAGCAGTGGTATCAACGC30370.3642644245473682No Hit
GTTGATACCACTGCTTCCCATGTAC28880.3463930385554163No Hit
CTTCCCATGTACTCTGCGTTGATAC27250.32684246193334815No Hit
CCACTGCTTCCCATGTACTCTGCGT27140.3255230978668282No Hit
TATCAACGCAGAGTACTTTTTTTTT23760.28498263836830645No Hit
CCATGTACTCTGCGTTGATACCACT23650.28366327430178656No Hit
AAGCAGTGGTATCAACGCAGAGTAC22900.27466761021187786No Hit
ATGGGAAGCAGTGGTATCAACGCAG22140.2655520039341038No Hit
TGATACCACTGCTTCCCATGTACTC20420.24492194762124656No Hit
TTCCCATGTACTCTGCGTTGATACC19640.23556645696774153No Hit
ATCAACGCAGAGTACATGGGAAGCA18630.22345229599333122No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA13980.1676791786358975No Hit
GTACATGGTAAGCAGTGGTATCAAC13010.15604478641294894No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12870.15436559578283268No Hit
GGAAGCAGTGGTATCAACGCAGAGT12720.15256646296485094No Hit
ACCATGTACTCTGCGTTGATACCAC10890.1306170425854738No Hit
CTGCGTTGATACCACTGCTTCCCAT9680.11610403785375449No Hit
AAAAAGTACTCTGCGTTGATACCAC9330.11190606127846378No Hit
TCAACGCAGAGTACATGGGAAGCAG9270.11118640815127108No Hit
CTCTGCGTTGATACCACTGCTTCCC8970.10758814251530763No Hit
TGGGAAGCAGTGGTATCAACGCAGA8820.1057890096973259No Hit
TCCATGTACTCTGCGTTGATACCAC8700.1043497034429405No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8600.10315028156428602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTGT350.002171948616.2824736
ACCGTGT602.5702584E-514.2471658
CAAGACT500.001498742413.2997484
CTCCCGG604.087067E-412.66718714
GTTCTAT550.003039353812.1052121
GTTTAAG550.003039353812.1052121
GTGATAA550.003039353812.1052121
CTGTGCA550.00307096612.0885029
CGCAAAA802.8631763E-511.8747752
GTAAAAA2700.011.6248481
GTGTAGG600.005820322311.0964451
CGACTCT600.00586998411.08378914
GTACAAA1803.45608E-1110.5680431
CCGGGTT750.002644465510.13374917
TCGACTC750.00264710110.13253313
GAACAAA3600.09.7754391
ACGCAAA1002.718903E-49.511241
ACTTACA800.00451244239.499822
TCAGAAG800.00451244239.499823
CAGTACT1304.2290485E-69.499824
Produced by FastQC (version 0.11.5)