FastQCFastQC Report
Wed 25 May 2016
SRR1294950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833735
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC293053.5149058153969786No Hit
GTACATGGGAAGCAGTGGTATCAAC284213.4088769213239214No Hit
GTATCAACGCAGAGTACTTTTTTTT147911.774064900717854No Hit
GAGTACATGGGAAGCAGTGGTATCA134931.6183799408685016No Hit
GGTATCAACGCAGAGTACTTTTTTT111221.3339970134395223No Hit
CATGTACTCTGCGTTGATACCACTG108591.302452218030909No Hit
GCGTTGATACCACTGCTTCCCATGT95201.1418496284790731No Hit
TATCAACGCAGAGTACTTTTTTTTT93141.1171415377787905No Hit
ACGCAGAGTACATGGGAAGCAGTGG92221.1061068564951693No Hit
TATCAACGCAGAGTACATGGGAAGC81100.9727311435887903No Hit
GTATCAACGCAGAGTACATGGGAAG80610.9668539763833831No Hit
GGTATCAACGCAGAGTACATGGGAA78810.9452643825676024No Hit
ACTCTGCGTTGATACCACTGCTTCC74780.8969276808578265No Hit
GTGGTATCAACGCAGAGTACATGGG54970.6593222066963724No Hit
GCTTCCCATGTACTCTGCGTTGATA53800.6452889707161148No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53020.6359334800626097No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46020.5519739485567956No Hit
GTACTCTGCGTTGATACCACTGCTT44030.5281054531715713No Hit
ATACCACTGCTTCCCATGTACTCTG41700.5001589233989218No Hit
GATACCACTGCTTCCCATGTACTCT40420.48480632335214424No Hit
CAGTGGTATCAACGCAGAGTACATG38590.46285690297276716No Hit
GGGAAGCAGTGGTATCAACGCAGAG38390.4604580592154582No Hit
CATGGGAAGCAGTGGTATCAACGCA38390.4604580592154582No Hit
CTGCTTCCCATGTACTCTGCGTTGA35490.42567482473447793No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35050.4203973684683982No Hit
GCAGTGGTATCAACGCAGAGTACAT33720.40444505748229354No Hit
GCAGAGTACATGGGAAGCAGTGGTA33700.4042051731065626No Hit
ACATGGGAAGCAGTGGTATCAACGC32850.3940100871379995No Hit
TACCACTGCTTCCCATGTACTCTGC32290.38729332461753435No Hit
CTTCCCATGTACTCTGCGTTGATAC29800.3574277198390376No Hit
GTTGATACCACTGCTTCCCATGTAC28550.3424349463558565No Hit
CCACTGCTTCCCATGTACTCTGCGT28060.3365577791504495No Hit
CCATGTACTCTGCGTTGATACCACT26580.31880633534636305No Hit
AAGCAGTGGTATCAACGCAGAGTAC24230.2906199211979826No Hit
ATGGGAAGCAGTGGTATCAACGCAG24220.29049997901011715No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22400.26867050081860544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22030.26423263986758383No Hit
TGATACCACTGCTTCCCATGTACTC20920.250919057014519No Hit
GTGGTATCAACGCAGAGTACTTTTT20310.2436025835547266No Hit
TTCCCATGTACTCTGCGTTGATACC20130.24144362417314855No Hit
ATCAACGCAGAGTACATGGGAAGCA20030.24024420229449406No Hit
ATCAACGCAGAGTACTTTTTTTTTT15540.18639015994290753No Hit
GTACATGGTAAGCAGTGGTATCAAC13920.1669595255087048No Hit
GGAAGCAGTGGTATCAACGCAGAGT13650.1637210864363377No Hit
ACCATGTACTCTGCGTTGATACCAC11010.13205634883985917No Hit
CTGCGTTGATACCACTGCTTCCCAT10240.12282080037421962No Hit
AAAAAGTACTCTGCGTTGATACCAC10100.12114160974410335No Hit
TCAACGCAGAGTACATGGGAAGCAG9860.11826299723533257No Hit
CTCTGCGTTGATACCACTGCTTCCC9730.11670374879308174No Hit
TCCATGTACTCTGCGTTGATACCAC9630.11550432691442725No Hit
CCCCATGTACTCTGCGTTGATACCA8980.10770808470317308No Hit
TGGGAAGCAGTGGTATCAACGCAGA8960.10746820032744217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA551.9555548E-413.82058052
GTGTACG500.001491840513.30789851
GGAACGA500.001499278913.29911815
AGACTGT604.088709E-412.6665876
GTCTAAT550.003052790212.0980881
GTTCTGA600.00584589511.0899141
GTCCTAG600.00584589511.0899141
CTATATT600.00586944211.0839284
GTGTACA803.7461138E-410.6938471
GGGTAAG1659.258656E-1010.3629497
TATACTT856.586498E-410.058765
GTCTTGG951.631941E-410.0059381
GAACAAA2800.09.8451291
TCAGGCC800.00450974669.5005093
GAACAAG1252.729001E-59.1254161
ATATACT1054.483496E-49.0481044
GTGTTGG950.00181179879.0053441
TGGCCTG850.0074291258.941125
CGGAATG1252.7402584E-48.35944614
CCCTGTA1050.0045004038.1442712
Produced by FastQC (version 0.11.5)