FastQCFastQC Report
Wed 25 May 2016
SRR1294949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867665
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC96001.1064177994963493No Hit
CCCATGTACTCTGCGTTGATACCAC89691.0336938795502872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70550.8131018307757026No Hit
GAGTACATGGGAAGCAGTGGTATCA44490.5127554989540895No Hit
CATGTACTCTGCGTTGATACCACTG33180.3824056519509258No Hit
GTATCAACGCAGAGTACTTTTTTTT30900.35612822921288745No Hit
GCGTTGATACCACTGCTTCCCATGT30450.35094189577774837No Hit
ACGCAGAGTACATGGGAAGCAGTGG27330.31498331729411694No Hit
GTATCAACGCAGAGTACATGGGAAG27070.3119867690871477No Hit
TATCAACGCAGAGTACATGGGAAGC26160.3014988503627552No Hit
GGTATCAACGCAGAGTACATGGGAA25310.29170244276304796No Hit
ACTCTGCGTTGATACCACTGCTTCC23370.2693435830648926No Hit
GTGGTATCAACGCAGAGTACATGGG22660.26116070142278414No Hit
GGTATCAACGCAGAGTACTTTTTTT22660.26116070142278414No Hit
TATCAACGCAGAGTACTTTTTTTTT17800.20514830032328144No Hit
GCTTCCCATGTACTCTGCGTTGATA16120.18578598883209535No Hit
GTACTCTGCGTTGATACCACTGCTT15190.17506756639947446No Hit
ATACCACTGCTTCCCATGTACTCTG13590.15662726974120197No Hit
GATACCACTGCTTCCCATGTACTCT12810.14763762512029413No Hit
CAGTGGTATCAACGCAGAGTACATG12290.14164452870635555No Hit
GGGAAGCAGTGGTATCAACGCAGAG12030.1386479804993863No Hit
CATGGGAAGCAGTGGTATCAACGCA11040.12723804694208019No Hit
GCAGAGTACATGGGAAGCAGTGGTA11010.12689229137973757No Hit
CTGCTTCCCATGTACTCTGCGTTGA10830.12481775800568193No Hit
GCAGTGGTATCAACGCAGAGTACAT10710.12343473575631148No Hit
TACCACTGCTTCCCATGTACTCTGC10180.1173263874882587No Hit
GTTGATACCACTGCTTCCCATGTAC9700.11179429849077697No Hit
GGTATCAACGCAGAGTACATGGGGG9500.10948926140849291No Hit
ACATGGGAAGCAGTGGTATCAACGC9430.10868249842969349No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9050.10430292797335378No Hit
CTTCCCATGTACTCTGCGTTGATAC9040.10418767611923957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA402.7331273E-416.645821
ACTAGTC456.747178E-414.780923
TCAGTAC400.00526917614.253033
CTTCTAG751.45791255E-512.6825291
AGTTAAG751.4797051E-512.66424510
GTACATA951.0224248E-612.0150281
CATAGTA1157.035669E-811.5676763
CTAGACA752.0677125E-411.4024234
TTATTCT855.3048338E-511.1788464
TCTAGGT600.005862822311.085693
CTAATCC600.005862822311.085693
TCTACAC700.0014876110.859453
TCTATCA700.0014876110.859452
GTCTTGG803.723725E-410.7008841
CAAGATA1355.6528734E-810.5577984
GTAAAAA2001.8189894E-1210.4630871
CCATAGT1303.8202234E-710.23294452
GGCCTAA1409.6864824E-810.17662410
GTGTAAA856.511309E-410.0714211
CTAGAGC951.6384014E-410.0021263
Produced by FastQC (version 0.11.5)