FastQCFastQC Report
Wed 25 May 2016
SRR1294949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867665
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC100331.1563218523277994No Hit
GTATCAACGCAGAGTACTTTTTTTT98581.1361527778578138No Hit
GTACATGGGAAGCAGTGGTATCAAC94851.0931638362732161No Hit
GGTATCAACGCAGAGTACTTTTTTT70490.8124103196510173No Hit
TATCAACGCAGAGTACTTTTTTTTT59610.6870163023747645No Hit
GAGTACATGGGAAGCAGTGGTATCA42750.49270167633821804No Hit
CATGTACTCTGCGTTGATACCACTG36070.4157134377899305No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34170.39381558550823187No Hit
GCGTTGATACCACTGCTTCCCATGT31670.36500262197968114No Hit
ACGCAGAGTACATGGGAAGCAGTGG30140.3473690883002081No Hit
GTATCAACGCAGAGTACATGGGAAG26730.30806820604726476No Hit
TATCAACGCAGAGTACATGGGAAGC26450.3048411541320671No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25320.29181769461716217No Hit
ACTCTGCGTTGATACCACTGCTTCC25170.2900889168054491No Hit
GGTATCAACGCAGAGTACATGGGAA24640.28398056853739634No Hit
GTGGTATCAACGCAGAGTACATGGG21220.2445644344303389No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19870.22900543412492147No Hit
GCTTCCCATGTACTCTGCGTTGATA17250.1988094483470003No Hit
GTACTCTGCGTTGATACCACTGCTT14520.16734569217382286No Hit
ATACCACTGCTTCCCATGTACTCTG14270.16446439582096778No Hit
GATACCACTGCTTCCCATGTACTCT13620.15697302530354457No Hit
CATGGGAAGCAGTGGTATCAACGCA12910.14879014366143614No Hit
GCAGAGTACTTTTTTTTTTTTTTTT12840.14798338068263672No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA12670.1460240991626953No Hit
GGGAAGCAGTGGTATCAACGCAGAG12650.14579359545446688No Hit
CAGTGGTATCAACGCAGAGTACATG12590.14510208432978164No Hit
GTGGTATCAACGCAGAGTACTTTTT12320.14199028426869817No Hit
GCAGAGTACATGGGAAGCAGTGGTA11490.13242438037721932No Hit
CTGCTTCCCATGTACTCTGCGTTGA11290.13011934329493524No Hit
TACCACTGCTTCCCATGTACTCTGC11220.12931258031613585No Hit
GCAGTGGTATCAACGCAGAGTACAT10870.12527876542213873No Hit
ATCAACGCAGAGTACTTTTTTTTTT10530.12136020238225584No Hit
ACATGGGAAGCAGTGGTATCAACGC10300.11870940973762915No Hit
GGTATCAACGCAGAGTACATGGGGG9730.11214005405311958No Hit
GTTGATACCACTGCTTCCCATGTAC9680.11156379478254858No Hit
CCACTGCTTCCCATGTACTCTGCGT9400.10833674286735089No Hit
CTTCCCATGTACTCTGCGTTGATAC9390.10822149101323668No Hit
CCATGTACTCTGCGTTGATACCACT8910.10268940201575492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGC1006.5847416E-1014.2499143
TAGAGCT1400.014.2499124
GCGTCTC400.005278846714.2490929
CTTAGCC500.001498685213.299923
AGACCGT655.4473854E-513.1537666
GTATATA604.0652003E-412.6753551
GGTCTAG550.003050857712.0992031
GTATAGG951.0294407E-612.0082321
GTATTGG802.8429176E-511.8831461
TTACACA752.071919E-411.3999314
TTTAGGC855.316676E-511.1764023
ACTAACT600.00587489811.0826288
CCCATAG951.3478797E-511.0075451
TATAACT951.3582572E-510.9999334
AGTACTC1502.1245796E-910.7666025
ACCTAGA909.43719E-510.5627971
GTCTTGG1355.613765E-810.5627971
GTAAATT909.5091826E-510.55488315
CCTTAGC1002.3906168E-510.4517432
GTTTAGA1105.9849226E-610.3707461
Produced by FastQC (version 0.11.5)