FastQCFastQC Report
Wed 25 May 2016
SRR1294948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233333
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC75070.17733072262446634No Hit
CCCATGTACTCTGCGTTGATACCAC69830.1649527688939188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCA1502.1445885E-910.764439
GTCTTAG3750.09.6408491
GTCCTAG4500.09.5139951
GGACCGT900.00111622649.4982516
GTCCTAA3700.09.2568611
TACACCG850.00744247868.9406925
GGACGTC850.0074504218.939536
TGGACCG1304.375162E-58.7687565
GTACTAG2950.08.7077251
CTGTCCG1107.1635307E-48.6345699
GTCTTGG7250.08.5297891
GTATTAG6250.08.524541
TAGACAG6400.08.460485
TTATACT8050.08.3799144
TAGGACC2855.456968E-128.334364
CTAGGAC4800.08.3135243
TAAGGTG4000.08.312055
TAGACCG1150.00111183288.2604225
GCACCGT1502.5942121E-58.2318176
GTCTTAA5450.08.0301621
Produced by FastQC (version 0.11.5)