FastQCFastQC Report
Wed 25 May 2016
SRR1294948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233333
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94080.22223623797135733No Hit
CCCATGTACTCTGCGTTGATACCAC76470.18063780949904013No Hit
GTACATGGGAAGCAGTGGTATCAAC72290.170763792973527No Hit
GGTATCAACGCAGAGTACTTTTTTT54510.12876378966644011No Hit
TATCAACGCAGAGTACTTTTTTTTT54250.12814961638973357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG600.00587430711.0843945
ACTACCG750.002647081610.1343035
CCGATCA750.002651494710.1322689
CTAGGAC3900.09.9883723
GACGCTC900.001115429.499001511
TCTATAC5250.09.4106473
GGACCGT1451.7060047E-69.1732926
TATACCG1151.1103334E-49.0878275
CTGTCCG1151.11276444E-49.0860029
ACCGTTC1151.11276444E-49.0860028
GCTCGTT1151.11276444E-49.08600211
TGGACCG1054.4912196E-49.0484855
ACAACGC1054.5002432E-49.0466688
GTCCTAC2750.08.9880931
GTATTAG6050.08.9566661
CTAGTAC2701.8189894E-128.7973473
CAGGACT6700.08.7918864
CCGTGCA1651.0527656E-68.6354569
TTCGTGA1107.156218E-48.63545610
AGTACCG1000.0029040258.5508195
Produced by FastQC (version 0.11.5)