FastQCFastQC Report
Wed 25 May 2016
SRR1294947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4017268
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG909.5185285E-510.5560195
ATACCGT750.00265337410.1313796
TAGACAG6050.09.8929975
GTCCTAA3850.09.6377321
TGTGCGA800.0045252689.49816910
TGTACCG1252.7549784E-59.12045
TAGGGTG4700.09.0961445
TACCGTG1151.1137899E-49.0852047
GTATCAA42700.08.868011
CAGGACT5700.08.8342724
GTATTAT8100.08.5744991
TGCGTTG11200.08.56531212
GCGTCTT1000.00290859848.54931115
GTACCGT1000.0029115528.5483526
GTCCTAG4900.08.5433371
GTATAGA5600.08.4947951
AAACCGT1356.818448E-58.4428176
GTCTTAG3300.08.3609381
CCTACAC4250.08.2714673
CTGCGTT11600.08.18817811
Produced by FastQC (version 0.11.5)