FastQCFastQC Report
Wed 25 May 2016
SRR1294947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4017268
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105390.26234246756751106No Hit
TATCAACGCAGAGTACTTTTTTTTT56810.14141451354502613No Hit
GGTATCAACGCAGAGTACTTTTTTT56750.14126515831156897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG604.0934733E-412.6670574
ACCGATC550.003071769812.0899278
TTACGCT700.001491910210.8574774
TGGGCGC1106.0576403E-610.36279510
GTTAGAC1803.947207E-1010.0280863
GTATCAA50700.09.3378091
ACTGCGT1356.860955E-69.1474058
GTCTAGG5050.08.8476931
CTGTCGC1951.6536433E-88.7685189
GTATTAA8250.08.7575631
GTCCTAC2304.0017767E-108.6799071
CTACGAC1252.7441964E-48.3602573
TATTCCG1606.4529213E-68.3127565
TCGCCCA2409.349606E-108.31182512
TGTCGCC2409.349606E-108.31182510
AGCGCTG1150.00111086788.2611245
TACACTG8800.08.2047985
GTCGCCC2201.46828825E-88.20387911
AAGACCG1050.004513828.1431075
TGCGCAG1050.00451837578.14219610
Produced by FastQC (version 0.11.5)