FastQCFastQC Report
Wed 25 May 2016
SRR1294946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679715
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC52740.14332631739142843No Hit
CCCATGTACTCTGCGTTGATACCAC47690.12960242844894238No Hit
GTATCAACGCAGAGTACTTTTTTTT45950.12487380136776897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGC550.003074065512.0886766
TACGACC700.001490984910.8582394
GTACGAC700.001490984910.8582393
AGGACCG803.7740628E-410.6872715
ACGCTGC803.779759E-410.6855276
CTTACGC856.591388E-410.0598393
ACAGTCG800.0045248139.4982468
GCGTGCG850.0074500678.9395269
GTCTAAG2950.08.70704651
GTATTAA7800.08.6595021
CTAGGAC3950.08.6591023
GTCTAGG3850.08.6484141
TAACCCG1356.804327E-58.44426255
TCTATAC6100.08.4106863
TAGGACC2606.002665E-118.4046954
TAACACG1150.0011100418.26170354
CCGCTTG1150.00111318478.2593439
GTCCTAC2658.731149E-118.2567861
GTATCAA44950.08.2328271
GTATAGG4300.08.1858231
Produced by FastQC (version 0.11.5)