FastQCFastQC Report
Wed 25 May 2016
SRR1294946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679715
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT123450.33548793860394077No Hit
GGTATCAACGCAGAGTACTTTTTTT68570.18634595342302326No Hit
TATCAACGCAGAGTACTTTTTTTTT64330.17482332191487657No Hit
CCCATGTACTCTGCGTTGATACCAC51540.14006519526648123No Hit
GTACATGGGAAGCAGTGGTATCAAC51180.13908685862899708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA350.002172193316.2843289
CGTTCCG855.3318367E-511.17551910
GCTATAC2400.09.8963363
ACCGTCT800.00452110039.4991918
GTATCAA57350.09.0010981
CACCGTC950.00182610568.9992347
CTAGACG850.0074356938.941634
ACCGTTT850.00744398548.94041512
GACAGCG1502.7072947E-68.8659127
CTAGACT3550.08.8314354
CCGAGCA1201.7665453E-48.7075929
TGTACCG1000.00290502148.5504345
GTATTAG6600.08.4983991
ATAGGAC2803.6379788E-128.48257353
CGAATAG1356.809391E-58.44372615
TAGACAG6550.08.4126415
ATTAGAC3550.08.2961963
TTAGGAC3450.08.2612893
ACCGTCC1150.00111208798.2601668
TTAGGCA4550.08.14327054
Produced by FastQC (version 0.11.5)