FastQCFastQC Report
Wed 25 May 2016
SRR1294945_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294945_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424259
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC98170.2866897626610604No Hit
CCCATGTACTCTGCGTTGATACCAC88010.2570191098278489No Hit
GAGTACATGGGAAGCAGTGGTATCA43330.1265383255180172No Hit
GTATCAACGCAGAGTACTTTTTTTT42640.12452329102442308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG604.0939412E-412.6667835
AGCCGCG1850.012.3262412
AGCCTCG3600.011.6129162
GCCTAAC1303.205423E-810.9765611
GCCGCGG1909.094947E-1210.5016323
TATACCG951.6449808E-410.00009255
TAGACAG4800.09.5000885
TGGACCG1106.840451E-59.5000885
TATACTG8300.09.3856285
TAGACTG4500.09.2889755
CCTAGAC2800.09.1621373
TTAGACT3150.09.0491574
CGTTCCG950.00182447129.0000835
CTCGGCG950.00182447129.0000835
GGACCGT950.00182835718.9979796
CTGGTCG850.00745139138.9392999
GGACCGA850.00745228458.9391696
TTATACT6700.08.9343554
GCCTCGG4500.08.8680453
GGGTAGC1651.0304047E-68.6481991
Produced by FastQC (version 0.11.5)