FastQCFastQC Report
Wed 25 May 2016
SRR1294945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424259
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106840.312009109124047No Hit
GTACATGGGAAGCAGTGGTATCAAC97590.28499596555050305No Hit
CCCATGTACTCTGCGTTGATACCAC94500.2759721154270165No Hit
GGTATCAACGCAGAGTACTTTTTTT65810.19218756525134342No Hit
TATCAACGCAGAGTACTTTTTTTTT60090.17548322133343303No Hit
GAGTACATGGGAAGCAGTGGTATCA42920.1253409861812439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG905.405527E-712.6677875
CGTACAC658.0208154E-411.6935133
GTCTAGG3150.011.1664031
CGTCAAG600.00588254711.0822112
AGAGCGC700.001493459610.8560428
CAGTGCG856.603418E-410.0578039
GTATTAG5150.09.78342151
AACAGCG1304.2477586E-69.4990377
GTATTAA7100.09.2387431
CCTGGCG850.0074322918.9420983
TCTAGAC3350.08.791953
CGGGTTC1856.5923814E-88.72884418
GTCTAGA4150.08.7047771
CTAGCAC3550.08.5642623
GTACTAG2951.8189894E-128.3786381
GTATTAC3850.08.1484561
ACTGTCC4100.08.1089338
GTATTAT7300.08.0740411
GTCTATG3550.08.03368951
TAGACAG6150.08.033235
Produced by FastQC (version 0.11.5)