FastQCFastQC Report
Wed 25 May 2016
SRR1294944_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294944_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5407325
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC106580.197103003795777No Hit
CCCATGTACTCTGCGTTGATACCAC94240.17428210806637293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACC750.002652814610.1317548
CTTACCG750.002652814610.1317549
TAGACCG1151.11173766E-49.0868855
TACGGCT1054.492224E-49.0483864
GTCCTGG11400.08.92734051
TACACCG1401.086448E-58.821365
TATACTG11500.08.6738455
CTAAGAC4850.08.6191273
CGCGATC1554.2137126E-68.5804217
GTATTAG8100.08.5719771
TAAGACA9100.08.456764
TGAACCG1252.745799E-48.3599355
TCTGTCG4100.08.3401638
TAGACAG8550.08.3332685
CTGTCGC4450.08.3245459
GTCCTAC4800.08.322451
ACCGACT1150.00111307728.2595828
CCTACAC7300.08.1991733
TCCGTAC1401.0392477E-48.1433963
CGTGCAA1050.00452155068.14166410
Produced by FastQC (version 0.11.5)