FastQCFastQC Report
Wed 25 May 2016
SRR1294944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5407325
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT131620.24341055882529716No Hit
CCCATGTACTCTGCGTTGATACCAC105640.19536462113891803No Hit
GTACATGGGAAGCAGTGGTATCAAC101030.18683914874730112No Hit
GGTATCAACGCAGAGTACTTTTTTT72280.13367053025294393No Hit
TATCAACGCAGAGTACTTTTTTTTT69920.1293060801782767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG8600.09.61092955
ACCGATC800.00452222549.4990538
CCTACGC850.00743566648.9417723
CTAAGCG1201.7640433E-48.7089153
TTACACT8100.08.5621414
CCTATAC6000.08.55057053
TTAGACA6450.08.5430474
ATTAGAC4350.08.517813
GTCTAGG5650.08.4131641
TACTCCG1701.6126742E-68.3827575
TAATACT11050.08.3397684
GTACTAA3800.08.2559721
ACCGAGT1502.5919466E-58.23251158
GTATTAA9550.08.0634241
GTGTTAG8650.08.0231441
GTATCAA78600.07.97077751
AGGACCG1553.899522E-57.9681265
GGACAGT9250.07.9084896
GTATAAA12200.07.87044671
GTCTTAT7250.07.86775971
Produced by FastQC (version 0.11.5)