FastQCFastQC Report
Wed 25 May 2016
SRR1294943_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294943_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161823
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC106650.25625789467740456No Hit
CCCATGTACTCTGCGTTGATACCAC97350.233911917926351No Hit
GTATCAACGCAGAGTACTTTTTTTT48240.11591074392159398No Hit
GAGTACATGGGAAGCAGTGGTATCA46430.11156168823133515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA1201.5290098E-610.28984311
CAATCCG1201.5290098E-610.28984310
ACAGCGC856.610114E-410.0567998
GAGTACG800.0044671659.5130751
TAACCCG1002.7572448E-49.500035
GTCTAGG3550.09.1111141
GTCCTAA4000.09.037421
GTATTAG7600.09.0123871
ATAGGCG1304.3721662E-58.76925855
CCTACAC5250.08.686163
TTAGACA6350.08.6777144
CCAGTAC4500.08.6559993
TCTGTCG3100.08.57891758
CCTAGAC3550.08.563823
TAGGACA4950.08.444984
GTCTTAG5650.08.4186511
GTGTTAG6050.08.3337681
TAGACTG6050.08.3223415
CTGTCGC3100.08.27242859
ATAGGAC3450.08.2612933
Produced by FastQC (version 0.11.5)